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Protocol for parallel proteomic and metabolomic analysis of mouse intervertebral disc tissues.
Veras, Matthew A; Lim, Yong J; Kuljanin, Miljan; Lajoie, Gilles A; Urquhart, Bradley L; Séguin, Cheryle A.
Afiliación
  • Veras MA; Department of Physiology and Pharmacology, Schulich School of Medicine & Dentistry The University of Western Ontario London Ontario Canada.
  • Lim YJ; Bone and Joint Institute The University of Western Ontario London Ontario Canada.
  • Kuljanin M; Department of Physiology and Pharmacology, Schulich School of Medicine & Dentistry The University of Western Ontario London Ontario Canada.
  • Lajoie GA; Department of Cell Biology Harvard Medical School Boston Massachusetts USA.
  • Urquhart BL; Department of Biochemistry, Schulich School of Medicine & Dentistry The University of Western Ontario London Ontario Canada.
  • Séguin CA; Department of Physiology and Pharmacology, Schulich School of Medicine & Dentistry The University of Western Ontario London Ontario Canada.
JOR Spine ; 3(3): e1099, 2020 Sep.
Article en En | MEDLINE | ID: mdl-33015574
The comprehensiveness of data collected by "omics" modalities has demonstrated the ability to drastically transform our understanding of the molecular mechanisms of chronic, complex diseases such as musculoskeletal pathologies, how biomarkers are identified, and how therapeutic targets are developed. Standardization of protocols will enable comparisons between findings reported by multiple research groups and move the application of these technologies forward. Herein, we describe a protocol for parallel proteomic and metabolomic analysis of mouse intervertebral disc (IVD) tissues, building from the combined expertise of our collaborative team. This protocol covers dissection of murine IVD tissues, sample isolation, and data analysis for both proteomics and metabolomics applications. The protocol presented below was optimized to maximize the utility of a mouse model for "omics" applications, accounting for the challenges associated with the small starting quantity of sample due to small tissue size as well as the extracellular matrix-rich nature of the tissue.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Guideline / Prognostic_studies Idioma: En Revista: JOR Spine Año: 2020 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Guideline / Prognostic_studies Idioma: En Revista: JOR Spine Año: 2020 Tipo del documento: Article Pais de publicación: Estados Unidos