Your browser doesn't support javascript.
loading
Nanofountain Probe Electroporation Enables Versatile Single-Cell Intracellular Delivery and Investigation of Postpulse Electropore Dynamics.
Nathamgari, Samba Shiva Prasad; Pathak, Nibir; Lemaitre, Vincent; Mukherjee, Prithvijit; Muldoon, Joseph J; Peng, Chian-Yu; McGuire, Tammy; Leonard, Joshua N; Kessler, John A; Espinosa, Horacio Dante.
Afiliación
  • Nathamgari SSP; Department of Mechanical Engineering, Northwestern University, Evanston, IL, 60208, USA.
  • Pathak N; Theoretical and Applied Mechanics Program, Northwestern University, Evanston, IL, 60208, USA.
  • Lemaitre V; Department of Mechanical Engineering, Northwestern University, Evanston, IL, 60208, USA.
  • Mukherjee P; Theoretical and Applied Mechanics Program, Northwestern University, Evanston, IL, 60208, USA.
  • Muldoon JJ; iNfinitesimal LLC, Skokie, IL, 60077, USA.
  • Peng CY; Department of Mechanical Engineering, Northwestern University, Evanston, IL, 60208, USA.
  • McGuire T; Theoretical and Applied Mechanics Program, Northwestern University, Evanston, IL, 60208, USA.
  • Leonard JN; Department of Chemical and Biological Engineering and Interdisciplinary Biological Sciences Program, Northwestern University, Evanston, IL, 60208, USA.
  • Kessler JA; Department of Neurology, Northwestern University Feinberg School of Medicine, Chicago, IL, 60611, USA.
  • Espinosa HD; Department of Neurology, Northwestern University Feinberg School of Medicine, Chicago, IL, 60611, USA.
Small ; 16(43): e2002616, 2020 10.
Article en En | MEDLINE | ID: mdl-33006271
Introducing exogenous molecules into cells with high efficiency and dosage control is a crucial step in basic research as well as clinical applications. Here, the capability of the nanofountain probe electroporation (NFP-E) system to deliver proteins and plasmids in a variety of continuous and primary cell types with appropriate dosage control is reported. It is shown that the NFP-E can achieve fine control over the relative expression of two cotransfected plasmids. Finally, the dynamics of electropore closure after the pulsing ends with the NFP-E is investigated. Localized electroporation has recently been utilized to demonstrate the converse process of delivery (sampling), in which a small volume of the cytosol is retrieved during electroporation without causing cell lysis. Single-cell temporal sampling confers the benefit of monitoring the same cell over time and can provide valuable insights into the mechanisms underlying processes such as stem cell differentiation and disease progression. NFP-E parameters that maximize the membrane resealing time, which is essential for increasing the sampled volume and in meeting the challenge of monitoring low copy number biomarkers, are identified. Its application in CRISPR/Cas9 gene editing, stem cell reprogramming, and single-cell sampling studies is envisioned.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Electroporación / Edición Génica Idioma: En Revista: Small Asunto de la revista: ENGENHARIA BIOMEDICA Año: 2020 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Alemania
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Electroporación / Edición Génica Idioma: En Revista: Small Asunto de la revista: ENGENHARIA BIOMEDICA Año: 2020 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Alemania