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In utero human intestine harbors unique metabolome, including bacterial metabolites.
Li, Yujia; Toothaker, Jessica M; Ben-Simon, Shira; Ozeri, Lital; Schweitzer, Ron; McCourt, Blake T; McCourt, Collin C; Werner, Lael; Snapper, Scott B; Shouval, Dror S; Khatib, Soliman; Koren, Omry; Agnihorti, Sameer; Tseng, George; Konnikova, Liza.
Afiliación
  • Li Y; Department of Biostatistics and.
  • Toothaker JM; Department of Immunology, University of Pittsburgh, Pittsburgh, Pennsylvania, USA.
  • Ben-Simon S; Azrieli Faculty of Medicine, Bar Ilan University, Safed, Israel.
  • Ozeri L; Azrieli Faculty of Medicine, Bar Ilan University, Safed, Israel.
  • Schweitzer R; Analytical Chemistry Laboratory, Tel-Hai College, Upper Galilee, Israel.
  • McCourt BT; Department of Pediatrics, Yale University, New Haven, Connecticut, USA.
  • McCourt CC; Department of Pediatrics, University of Pittsburgh, Pittsburgh, Pennsylvania, USA.
  • Werner L; Pediatric Gastroenterology Unit, Edmond and Lily Safra Children's Hospital, Sheba Medical Center, Ramat Gan, Israel.
  • Snapper SB; Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel.
  • Shouval DS; Division of Gastroenterology, Hepatology and Nutrition, Boston Children's Hospital, Boston, Massachusetts, USA.
  • Khatib S; Pediatric Gastroenterology Unit, Edmond and Lily Safra Children's Hospital, Sheba Medical Center, Ramat Gan, Israel.
  • Koren O; Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel.
  • Agnihorti S; Analytical Chemistry Laboratory, Tel-Hai College, Upper Galilee, Israel.
  • Tseng G; Department of Natural Compounds and Analytical Chemistry, Migal Galilee Research Institute, Kiryat Shmona, Israel.
  • Konnikova L; Azrieli Faculty of Medicine, Bar Ilan University, Safed, Israel.
JCI Insight ; 5(21)2020 11 05.
Article en En | MEDLINE | ID: mdl-33001863
Symbiotic microbial colonization through the establishment of the intestinal microbiome is critical to many intestinal functions, including nutrient metabolism, intestinal barrier integrity, and immune regulation. Recent studies suggest that education of intestinal immunity may be ongoing in utero. However, the drivers of this process are unknown. The microbiome and its byproducts are one potential source. Whether a fetal intestinal microbiome exists is controversial, and whether microbially derived metabolites are present in utero is unknown. Here, we aimed to determine whether bacterial DNA and microbially derived metabolites can be detected in second trimester human intestinal samples. Although we were unable to amplify bacterial DNA from fetal intestines, we report a fetal metabolomic intestinal profile with an abundance of bacterially derived and host-derived metabolites commonly produced in response to microbiota. Though we did not directly assess their source and function, we hypothesize that these microbial-associated metabolites either come from the maternal microbiome and are vertically transmitted to the fetus to prime the fetal immune system and prepare the gastrointestinal tract for postnatal microbial encounters or are produced locally by bacteria that were below our detection threshold.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Bacterias / Tracto Gastrointestinal / Metaboloma / Feto / Microbioma Gastrointestinal / Intestinos Límite: Adolescent / Child / Female / Humans / Infant / Male / Newborn Idioma: En Revista: JCI Insight Año: 2020 Tipo del documento: Article Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Bacterias / Tracto Gastrointestinal / Metaboloma / Feto / Microbioma Gastrointestinal / Intestinos Límite: Adolescent / Child / Female / Humans / Infant / Male / Newborn Idioma: En Revista: JCI Insight Año: 2020 Tipo del documento: Article Pais de publicación: Estados Unidos