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Production and characterization of monoclonal antibodies against highly immunogenic Opisthorchis viverrini proteins and development of coproantigen detection.
Arimatsu, Yuji; Teimoori, Salma; Surapaitoon, Arpa; Sripa, Banchob.
Afiliación
  • Arimatsu Y; WHO Collaborating Centre for Research and Control of Opisthorchiasis (Southeast Asian Liver Fluke Disease), Tropical Disease Research Center, Department of Pathology, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand.
  • Teimoori S; WHO Collaborating Centre for Research and Control of Opisthorchiasis (Southeast Asian Liver Fluke Disease), Tropical Disease Research Center, Department of Pathology, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand.
  • Surapaitoon A; WHO Collaborating Centre for Research and Control of Opisthorchiasis (Southeast Asian Liver Fluke Disease), Tropical Disease Research Center, Department of Pathology, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand.
  • Sripa B; WHO Collaborating Centre for Research and Control of Opisthorchiasis (Southeast Asian Liver Fluke Disease), Tropical Disease Research Center, Department of Pathology, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand. Electronic address: banchob@kku.ac.th.
Mol Biochem Parasitol ; 240: 111323, 2020 11.
Article en En | MEDLINE | ID: mdl-32980451
Opisthorchis viverrini and other foodborne trematode infections are major health concerns in the Greater Mekong Subregion. Currently, the gold-standard diagnostic method for opisthorchiasis is conventional stool examination for the presence of parasite eggs. This method lacks sensitivity and needs an experienced technician. We therefore produced monoclonal antibodies to highly immunogenic O. viverrini proteins aiming at detecting specific antigens in the feces. In this study, BALB/C mice were immunized using semi-purified somatic antigens and spleen cells were fused with a Sp2/0 myeloma cell line. Four hybridomas (1A2, 1E12, 2C7 and 8D6) were selected and cloned due to their strong reaction against O. viverrini somatic protein, resulting in three IgM clones and one IgG2 clone. Immunohistochemistry showed that 1A2, 1E12, 2C7 and 8D6 stained the parenchyma cells, gut, tegument and muscles, respectively. Western-blot analysis revealed that only antibody 1A2 could detect coproantigen (approx. 73 kDa protein) in feces of hamsters infected with O. viverrini. The 1A2 monoclonal antibody may be of value in the diagnosis of opisthorchiasis by coproantigen detection.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Opisthorchis / Inmunoensayo / Anticuerpos Antihelmínticos / Proteínas del Helminto / Anticuerpos Monoclonales / Antígenos Helmínticos Tipo de estudio: Diagnostic_studies Límite: Animals / Humans Idioma: En Revista: Mol Biochem Parasitol Año: 2020 Tipo del documento: Article País de afiliación: Tailandia Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Opisthorchis / Inmunoensayo / Anticuerpos Antihelmínticos / Proteínas del Helminto / Anticuerpos Monoclonales / Antígenos Helmínticos Tipo de estudio: Diagnostic_studies Límite: Animals / Humans Idioma: En Revista: Mol Biochem Parasitol Año: 2020 Tipo del documento: Article País de afiliación: Tailandia Pais de publicación: Países Bajos