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Designed Antimicrobial Peptides Against Trauma-Related Cutaneous Invasive Fungal Wound Infections.
Woodburn, Kathryn W; Jaynes, Jesse M; Clemens, L Edward.
Afiliación
  • Woodburn KW; Riptide Bioscience, Vallejo, CA 94592, USA.
  • Jaynes JM; Integrative Biosciences, Tuskegee University, Tuskegee, AL 36088, USA.
  • Clemens LE; Riptide Bioscience, Vallejo, CA 94592, USA.
J Fungi (Basel) ; 6(3)2020 Sep 22.
Article en En | MEDLINE | ID: mdl-32971819
Cutaneous invasive fungal wound infections after life-threatening dismounted complex blast injury (DCBI) and natural disasters complicate clinical care. These wounds often require aggressive repeated surgical debridement, can result in amputations and hemipelvectomies and have a 38% mortality rate. Given the substantial morbidity associated with cutaneous fungal wound infections, patients at risk need immediate empiric treatment mandating the use of rapidly acting broad-spectrum antimicrobials, acting on both fungi and bacteria, that are also effective against biofilm and can be administered topically. Designed antimicrobial peptides (dAMPs) are engineered analogues of innate antimicrobial peptides which provide the first line of defense against invading pathogens. The antifungal and antibacterial effect and mammalian cytotoxicity of seven innovative dAMPs, created by iterative structural analog revisions and physicochemical and functional testing were investigated. The dAMPs possess broad-spectrum antifungal activity, in addition to being effective against Gram-negative and Gram-positive bacteria, which is crucial as many wounds are polymicrobial and require immediate empiric treatment. Three of the most potent dAMPs-RP504, RP556 and RP557-possess limited mammalian cytotoxicity following 8 h incubation. If these encouraging broad-spectrum antimicrobial and rapid acting results are translated clinically, these novel dAMPs may become a first line empiric topical treatment for traumatic wound injuries.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: J Fungi (Basel) Año: 2020 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Suiza

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: J Fungi (Basel) Año: 2020 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Suiza