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CBF-1 Promotes the Establishment and Maintenance of HIV Latency by Recruiting Polycomb Repressive Complexes, PRC1 and PRC2, at HIV LTR.
Sharma, Adhikarimayum Lakhikumar; Hokello, Joseph; Sonti, Shilpa; Zicari, Sonia; Sun, Lin; Alqatawni, Aseel; Bukrinsky, Michael; Simon, Gary; Chauhan, Ashok; Daniel, Rene; Tyagi, Mudit.
Afiliación
  • Sharma AL; Center for Translational Medicine, Thomas Jefferson University, 1020 Locust Street, Philadelphia, PA 19107, USA.
  • Hokello J; Department of Basic Science, Faculty of Science and Technology, Kampala International University-Western Campus, P.O. Box 71, Bushenyi, Uganda.
  • Sonti S; Center for Translational Medicine, Thomas Jefferson University, 1020 Locust Street, Philadelphia, PA 19107, USA.
  • Zicari S; Section of Intercellular Interactions, Eunice Kennedy-Shriver National Institute of Child Health and Human Development, Bethesda, MD 20892, USA.
  • Sun L; Department of Pediatric Medicine, The Bambino Gesù Children's Hospital, 00165 Rome, Italy.
  • Alqatawni A; Division of Infectious Diseases, Department of Medicine, George Washington University, Washington, DC 20037, USA.
  • Bukrinsky M; Center for Translational Medicine, Thomas Jefferson University, 1020 Locust Street, Philadelphia, PA 19107, USA.
  • Simon G; Department of Microbiology, Immunology and Tropical Medicine, George Washington University, Washington, DC 20037, USA.
  • Chauhan A; Division of Infectious Diseases, Department of Medicine, George Washington University, Washington, DC 20037, USA.
  • Daniel R; Department of Pathology, Microbiology and Immunology, University of South Carolina School of Medicine, Columbia, SC 29209, USA.
  • Tyagi M; Center for Translational Medicine, Thomas Jefferson University, 1020 Locust Street, Philadelphia, PA 19107, USA.
Viruses ; 12(9)2020 09 18.
Article en En | MEDLINE | ID: mdl-32961937
The C-promoter binding factor-1 (CBF-1) is a potent and specific inhibitor of the human immunodeficiency virus (HIV)-1 LTR promoter. Here, we demonstrate that the knockdown of endogenous CBF-1 in latently infected primary CD4+ T cells, using specific small hairpin RNAs (shRNA), resulted in the reactivation of latent HIV proviruses. Chromatin immunoprecipitation (ChIP) assays using latently infected primary T cells and Jurkat T-cell lines demonstrated that CBF-1 induces the establishment and maintenance of HIV latency by recruiting polycomb group (PcG/PRC) corepressor complexes or polycomb repressive complexes 1 and 2 (PRC1 and PRC2). Knockdown of CBF-1 resulted in the dissociation of PRCs corepressor complexes enhancing the recruitment of RNA polymerase II (RNAP II) at HIV LTR. Knockdown of certain components of PRC1 and PRC2 also led to the reactivation of latent proviruses. Similarly, the treatment of latently infected primary CD4+ T cells with the PRC2/EZH2 inhibitor, 3-deazaneplanocin A (DZNep), led to their reactivation.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: VIH-1 / Latencia del Virus / Proteína de Unión a la Señal Recombinante J de las Inmunoglobulinas Límite: Humans Idioma: En Revista: Viruses Año: 2020 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Suiza
Texto completo
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XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: VIH-1 / Latencia del Virus / Proteína de Unión a la Señal Recombinante J de las Inmunoglobulinas Límite: Humans Idioma: En Revista: Viruses Año: 2020 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Suiza