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Metabolomic analysis of fibrotic mice combined with public RNA-Seq human lung data reveal potential diagnostic biomarker candidates for lung fibrosis.
Nojima, Yosui; Takeda, Yoshito; Maeda, Yohei; Bamba, Takeshi; Fukusaki, Eiichiro; Itoh, Mari N; Mizuguchi, Kenji; Kumanogoh, Atsushi.
Afiliación
  • Nojima Y; Laboratory of Bioinformatics, Artificial Intelligence Center for Health and Biomedical Research (ArCHER), National Institutes of Biomedical Innovation, Health and Nutrition, Osaka, Japan.
  • Takeda Y; Department of Respiratory Medicine and Clinical Immunology, Osaka University Graduate School of Medicine, Japan.
  • Maeda Y; Department of Respiratory Medicine and Clinical Immunology, Osaka University Graduate School of Medicine, Japan.
  • Bamba T; Department of Biotechnology, Graduate School of Engineering, Osaka University, Japan.
  • Fukusaki E; Division of Metabolomics, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan.
  • Itoh MN; Department of Biotechnology, Graduate School of Engineering, Osaka University, Japan.
  • Mizuguchi K; Laboratory of Bioinformatics, Artificial Intelligence Center for Health and Biomedical Research (ArCHER), National Institutes of Biomedical Innovation, Health and Nutrition, Osaka, Japan.
  • Kumanogoh A; Laboratory of Bioinformatics, Artificial Intelligence Center for Health and Biomedical Research (ArCHER), National Institutes of Biomedical Innovation, Health and Nutrition, Osaka, Japan.
FEBS Open Bio ; 10(11): 2427-2436, 2020 11.
Article en En | MEDLINE | ID: mdl-32961634
Idiopathic pulmonary fibrosis (IPF) is a severe lung disease with poor survival that warrants early and precise diagnosis for timely therapeutic intervention. Despite accumulating genomic, transcriptomic, proteomic, and lipidomic data on IPF, evidence from water-soluble metabolomics is limited. To identify biomarkers for IPF from water-soluble metabolomic data, we measured the levels of various metabolites in bronchoalveolar lavage fluid (BALF) and serum samples from a bleomycin-induced murine pulmonary fibrotic model using gas chromatography/mass spectrometry. Thirty-two of 73 BALF metabolites and 29 of 74 serum metabolites were annotated. We observed that the levels of proline and methionine were higher in BALF but lower in serum than those in the control. Furthermore, analysis of public RNA-Seq data from the lungs of patients with IPF revealed that proline- and methionine-related genes were significantly upregulated compared to those in the lungs of healthy controls. These results suggest that proline and methionine may be potential biomarkers for IPF and may help to deepen our understanding of the pathophysiology of the disease. Based on our results, we propose a model capable of recapitulating the proline and methionine metabolism of fibrotic lungs, thereby providing better means for studying the disease and developing novel therapeutic strategies for IPF.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fibrosis Pulmonar / Biomarcadores / Metabolómica / RNA-Seq / Pulmón Tipo de estudio: Diagnostic_studies / Prognostic_studies Límite: Animals / Humans Idioma: En Revista: FEBS Open Bio Año: 2020 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fibrosis Pulmonar / Biomarcadores / Metabolómica / RNA-Seq / Pulmón Tipo de estudio: Diagnostic_studies / Prognostic_studies Límite: Animals / Humans Idioma: En Revista: FEBS Open Bio Año: 2020 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Reino Unido