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Expression of Paracrine Effectors in Human Adipose-Derived Mesenchymal Stem Cells Treated With Plasma From Brown Bears (Ursus arctos).
Berg von Linde, Maria; Johansson, Karin; Kruse, Robert; Helenius, Gisela; Samano, Ninos; Friberg, Örjan; Frøbert, Anne Mette; Fröbert, Ole.
Afiliación
  • Berg von Linde M; Department of Cardiology, Faculty of Health, Örebro University, Örebro, Sweden.
  • Johansson K; Department of Laboratory Medicine, Faculty of Medicine and Health, Örebro University, Örebro, Sweden.
  • Kruse R; Department of Clinical Research Laboratory, Faculty of Medicine and Health, Örebro University, Örebro, Sweden.
  • Helenius G; iRiSC - Inflammatory Response and Infection Susceptibility Centre, Faculty of Medicine and Health, Örebro University, Örebro, Sweden.
  • Samano N; Department of Laboratory Medicine, Faculty of Medicine and Health, Örebro University, Örebro, Sweden.
  • Friberg Ö; Department of Cardiothoracic and Vascular Surgery, Faculty of Medicine and Health, University Health Care Research Center, Örebro University, Örebro, Sweden.
  • Frøbert AM; Department of Cardiothoracic and Vascular Surgery, Faculty of Medicine and Health, Örebro University, Örebro, Sweden.
  • Fröbert O; Department of Chemistry and Bioscience, Faculty of Engineering and Science, Aalborg University, Aalborg, Denmark.
Clin Transl Sci ; 14(1): 317-325, 2021 01.
Article en En | MEDLINE | ID: mdl-32949228
Adipose-derived mesenchymal stem cells (ADSCs) are promising candidates for novel cell therapeutic applications. Hibernating brown bears sustain tissue integrity and function via unknown mechanisms, which might be plasma borne. We hypothesized that plasma from hibernating bears may increase the expression of favorable factors from human ADSCs. In an experimental study, ADSCs from patients with ischemic heart disease were treated with interventional media containing plasma from hibernating and active bears, respectively, and with control medium. Extracted RNA from the ADSCs was sequenced using next generation sequencing. Statistical analyses of differentially expressed genes were performed using fold change analysis, pathway analysis, and gene ontology. As a result, we found that genes associated with inflammation, such as IGF1, PGF, IL11, and TGFA, were downregulated by > 10-fold in ADSCs treated with winter plasma compared with control. Genes important for cardiovascular development, ADM, ANGPTL4, and APOL3, were upregulated in ADSCs when treated with winter plasma compared with summer plasma. ADSCs treated with bear plasma, regardless if it was from hibernating or active bears, showed downregulation of IGF1, PGF, IL11, INHBA, IER3, and HMOX1 compared with control, suggesting reduced cell growth and differentiation. This can be summarized in the conclusion that plasma from hibernating bears suppresses inflammatory genes and activates genes associated with cardiovascular development in human ADSCs. Identifying the involved regulator(s) holds therapeutic potential.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Plasma / Ursidae / Isquemia Miocárdica / Trasplante de Células Madre Mesenquimatosas / Células Madre Mesenquimatosas Tipo de estudio: Prognostic_studies Límite: Aged / Aged80 / Animals / Female / Humans / Male Idioma: En Revista: Clin Transl Sci Año: 2021 Tipo del documento: Article País de afiliación: Suecia Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Plasma / Ursidae / Isquemia Miocárdica / Trasplante de Células Madre Mesenquimatosas / Células Madre Mesenquimatosas Tipo de estudio: Prognostic_studies Límite: Aged / Aged80 / Animals / Female / Humans / Male Idioma: En Revista: Clin Transl Sci Año: 2021 Tipo del documento: Article País de afiliación: Suecia Pais de publicación: Estados Unidos