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Cell Types of the Human Retina and Its Organoids at Single-Cell Resolution.
Cowan, Cameron S; Renner, Magdalena; De Gennaro, Martina; Gross-Scherf, Brigitte; Goldblum, David; Hou, Yanyan; Munz, Martin; Rodrigues, Tiago M; Krol, Jacek; Szikra, Tamas; Cuttat, Rachel; Waldt, Annick; Papasaikas, Panagiotis; Diggelmann, Roland; Patino-Alvarez, Claudia P; Galliker, Patricia; Spirig, Stefan E; Pavlinic, Dinko; Gerber-Hollbach, Nadine; Schuierer, Sven; Srdanovic, Aldin; Balogh, Marton; Panero, Riccardo; Kusnyerik, Akos; Szabo, Arnold; Stadler, Michael B; Orgül, Selim; Picelli, Simone; Hasler, Pascal W; Hierlemann, Andreas; Scholl, Hendrik P N; Roma, Guglielmo; Nigsch, Florian; Roska, Botond.
Afiliación
  • Cowan CS; Institute of Molecular and Clinical Ophthalmology Basel, 4031 Basel, Switzerland; Friedrich Miescher Institute for Biomedical Research, 4058 Basel, Switzerland.
  • Renner M; Institute of Molecular and Clinical Ophthalmology Basel, 4031 Basel, Switzerland; Friedrich Miescher Institute for Biomedical Research, 4058 Basel, Switzerland; Novartis Institutes for Biomedical Research, 4056 Basel, Switzerland.
  • De Gennaro M; Institute of Molecular and Clinical Ophthalmology Basel, 4031 Basel, Switzerland.
  • Gross-Scherf B; Institute of Molecular and Clinical Ophthalmology Basel, 4031 Basel, Switzerland; Friedrich Miescher Institute for Biomedical Research, 4058 Basel, Switzerland.
  • Goldblum D; Department of Ophthalmology, University of Basel, 4031 Basel, Switzerland.
  • Hou Y; Institute of Molecular and Clinical Ophthalmology Basel, 4031 Basel, Switzerland.
  • Munz M; Institute of Molecular and Clinical Ophthalmology Basel, 4031 Basel, Switzerland; Friedrich Miescher Institute for Biomedical Research, 4058 Basel, Switzerland.
  • Rodrigues TM; Institute of Molecular and Clinical Ophthalmology Basel, 4031 Basel, Switzerland.
  • Krol J; Institute of Molecular and Clinical Ophthalmology Basel, 4031 Basel, Switzerland; Friedrich Miescher Institute for Biomedical Research, 4058 Basel, Switzerland.
  • Szikra T; Institute of Molecular and Clinical Ophthalmology Basel, 4031 Basel, Switzerland; Friedrich Miescher Institute for Biomedical Research, 4058 Basel, Switzerland.
  • Cuttat R; Novartis Institutes for Biomedical Research, 4056 Basel, Switzerland.
  • Waldt A; Novartis Institutes for Biomedical Research, 4056 Basel, Switzerland.
  • Papasaikas P; Friedrich Miescher Institute for Biomedical Research, 4058 Basel, Switzerland; Swiss Institute of Bioinformatics, 4058 Basel, Switzerland.
  • Diggelmann R; Bio Engineering Laboratory, Department of Biosystems Science and Engineering of ETH Zurich, 4058 Basel, Switzerland.
  • Patino-Alvarez CP; Institute of Molecular and Clinical Ophthalmology Basel, 4031 Basel, Switzerland; Friedrich Miescher Institute for Biomedical Research, 4058 Basel, Switzerland.
  • Galliker P; Institute of Molecular and Clinical Ophthalmology Basel, 4031 Basel, Switzerland.
  • Spirig SE; Institute of Molecular and Clinical Ophthalmology Basel, 4031 Basel, Switzerland.
  • Pavlinic D; Institute of Molecular and Clinical Ophthalmology Basel, 4031 Basel, Switzerland.
  • Gerber-Hollbach N; Department of Ophthalmology, University of Basel, 4031 Basel, Switzerland.
  • Schuierer S; Novartis Institutes for Biomedical Research, 4056 Basel, Switzerland.
  • Srdanovic A; Friedrich Miescher Institute for Biomedical Research, 4058 Basel, Switzerland.
  • Balogh M; Institute of Molecular and Clinical Ophthalmology Basel, 4031 Basel, Switzerland.
  • Panero R; Institute of Molecular and Clinical Ophthalmology Basel, 4031 Basel, Switzerland.
  • Kusnyerik A; Institute of Molecular and Clinical Ophthalmology Basel, 4031 Basel, Switzerland; Department of Ophthalmology, Semmelweis University, 1085 Budapest, Hungary.
  • Szabo A; Department of Anatomy, Histology and Embryology, Semmelweis University, 1085 Budapest, Hungary.
  • Stadler MB; Friedrich Miescher Institute for Biomedical Research, 4058 Basel, Switzerland; Swiss Institute of Bioinformatics, 4058 Basel, Switzerland.
  • Orgül S; Department of Ophthalmology, University of Basel, 4031 Basel, Switzerland.
  • Picelli S; Institute of Molecular and Clinical Ophthalmology Basel, 4031 Basel, Switzerland.
  • Hasler PW; Department of Ophthalmology, University of Basel, 4031 Basel, Switzerland.
  • Hierlemann A; Bio Engineering Laboratory, Department of Biosystems Science and Engineering of ETH Zurich, 4058 Basel, Switzerland.
  • Scholl HPN; Institute of Molecular and Clinical Ophthalmology Basel, 4031 Basel, Switzerland; Department of Ophthalmology, University of Basel, 4031 Basel, Switzerland; Wilmer Eye Institute, Johns Hopkins University, Baltimore, MD 21287, USA.
  • Roma G; Novartis Institutes for Biomedical Research, 4056 Basel, Switzerland. Electronic address: guglielmo.roma@novartis.com.
  • Nigsch F; Novartis Institutes for Biomedical Research, 4056 Basel, Switzerland. Electronic address: florian.nigsch@novartis.com.
  • Roska B; Institute of Molecular and Clinical Ophthalmology Basel, 4031 Basel, Switzerland; Friedrich Miescher Institute for Biomedical Research, 4058 Basel, Switzerland; Department of Ophthalmology, University of Basel, 4031 Basel, Switzerland. Electronic address: botond.roska@iob.ch.
Cell ; 182(6): 1623-1640.e34, 2020 09 17.
Article en En | MEDLINE | ID: mdl-32946783
Human organoids recapitulating the cell-type diversity and function of their target organ are valuable for basic and translational research. We developed light-sensitive human retinal organoids with multiple nuclear and synaptic layers and functional synapses. We sequenced the RNA of 285,441 single cells from these organoids at seven developmental time points and from the periphery, fovea, pigment epithelium and choroid of light-responsive adult human retinas, and performed histochemistry. Cell types in organoids matured in vitro to a stable "developed" state at a rate similar to human retina development in vivo. Transcriptomes of organoid cell types converged toward the transcriptomes of adult peripheral retinal cell types. Expression of disease-associated genes was cell-type-specific in adult retina, and cell-type specificity was retained in organoids. We implicate unexpected cell types in diseases such as macular degeneration. This resource identifies cellular targets for studying disease mechanisms in organoids and for targeted repair in human retinas.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Retina / Sinapsis / Organoides / Diferenciación Celular / Análisis de la Célula Individual / Transcriptoma Límite: Female / Humans Idioma: En Revista: Cell Año: 2020 Tipo del documento: Article País de afiliación: Suiza Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Retina / Sinapsis / Organoides / Diferenciación Celular / Análisis de la Célula Individual / Transcriptoma Límite: Female / Humans Idioma: En Revista: Cell Año: 2020 Tipo del documento: Article País de afiliación: Suiza Pais de publicación: Estados Unidos