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Production of Highly Active Recombinant Dermonecrotic Toxin of Bordetella Pertussis.
Stanek, Ondrej; Linhartova, Irena; Holubova, Jana; Bumba, Ladislav; Gardian, Zdenko; Malandra, Anna; Bockova, Barbora; Teruya, Shihono; Horiguchi, Yasuhiko; Osicka, Radim; Sebo, Peter.
Afiliación
  • Stanek O; Institute of Microbiology of the Czech Academy of Sciences, Videnska 1083, 142 20 Prague, Czech Republic.
  • Linhartova I; Institute of Microbiology of the Czech Academy of Sciences, Videnska 1083, 142 20 Prague, Czech Republic.
  • Holubova J; Institute of Microbiology of the Czech Academy of Sciences, Videnska 1083, 142 20 Prague, Czech Republic.
  • Bumba L; Institute of Microbiology of the Czech Academy of Sciences, Videnska 1083, 142 20 Prague, Czech Republic.
  • Gardian Z; Institute of Parasitology, Biology Centre, The Czech Academy of Sciences, Branisovská 31, 370 05 Ceské Budejovice, Czech Republic.
  • Malandra A; Institute of Microbiology of the Czech Academy of Sciences, Videnska 1083, 142 20 Prague, Czech Republic.
  • Bockova B; Institute of Microbiology of the Czech Academy of Sciences, Videnska 1083, 142 20 Prague, Czech Republic.
  • Teruya S; Department of Molecular Bacteriology, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka 565-0871, Japan.
  • Horiguchi Y; Department of Molecular Bacteriology, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka 565-0871, Japan.
  • Osicka R; Institute of Microbiology of the Czech Academy of Sciences, Videnska 1083, 142 20 Prague, Czech Republic.
  • Sebo P; Institute of Microbiology of the Czech Academy of Sciences, Videnska 1083, 142 20 Prague, Czech Republic.
Toxins (Basel) ; 12(9)2020 09 15.
Article en En | MEDLINE | ID: mdl-32942577
Pathogenic Bordetella bacteria release a neurotropic dermonecrotic toxin (DNT) that is endocytosed into animal cells and permanently activates the Rho family GTPases by polyamination or deamidation of the glutamine residues in their switch II regions (e.g., Gln63 of RhoA). DNT was found to enable high level colonization of the nasal cavity of pigs by B. bronchiseptica and the capacity of DNT to inhibit differentiation of nasal turbinate bone osteoblasts causes atrophic rhinitis in infected pigs. However, it remains unknown whether DNT plays any role also in virulence of the human pathogen B. pertussis and in pathogenesis of the whooping cough disease. We report a procedure for purification of large amounts of LPS-free recombinant DNT that exhibits a high biological activity on cells expressing the DNT receptors Cav3.1 and Cav3.2. Electron microscopy and single particle image analysis of negatively stained preparations revealed that the DNT molecule adopts a V-shaped structure with well-resolved protein domains. These results open the way to structure-function studies on DNT and its interactions with airway epithelial layers.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Bordetella pertussis / Factores de Virulencia de Bordetella / Transglutaminasas / Células Epiteliales Límite: Animals / Humans Idioma: En Revista: Toxins (Basel) Año: 2020 Tipo del documento: Article País de afiliación: República Checa Pais de publicación: Suiza
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Bordetella pertussis / Factores de Virulencia de Bordetella / Transglutaminasas / Células Epiteliales Límite: Animals / Humans Idioma: En Revista: Toxins (Basel) Año: 2020 Tipo del documento: Article País de afiliación: República Checa Pais de publicación: Suiza