Plasmodium vivax: in vitro growth and reinvasion in red blood cells of Aotus nancymai.
Exp Parasitol
; 66(2): 183-8, 1988 Aug.
Article
en En
| MEDLINE
| ID: mdl-3294025
Plasmodium vivax was maintained in experimentally infected Aotus nancymai. Positive monkeys were used as donors for culture material. After leucocyte removal with two different methods, including the classic CF11 method and a commercially available filter, parasites were grown under continuous shaking conditions in standard RPMI 1640, containing 20% human AB + serum. When mature schizonts were present, artificially induced reticulocytes from monkeys pretreated with the hemolytic drug phenylhydrazine HCl were added. Addition of reticulocytes and shaking were both necessary to realize a significant reinvasion under in vitro conditions. A strong positive correlation between the percentage of reticulocytes and in vitro invasion was demonstrated, and a preferential invasion into reticulocytes was demonstrated in vivo and in vitro using blood films stained with brilliant cresyl blue and counterstained with Giemsa.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Plasmodium vivax
/
Reticulocitos
/
Eritrocitos
Límite:
Animals
Idioma:
En
Revista:
Exp Parasitol
Año:
1988
Tipo del documento:
Article
País de afiliación:
Países Bajos
Pais de publicación:
Estados Unidos