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Tiron protects against nicotine-induced lung and liver injury through antioxidant and anti-inflammatory actions in rats in vivo.
Khaled, Shimaa; Makled, Mirhan N; Nader, Manar A.
Afiliación
  • Khaled S; Department of Pharmacology and Toxicology, Faculty of Pharmacy, Mansoura University, Mansoura, Egypt; Department of Pharmacology and Toxicology, Faculty of Pharmacy, Horus University, Egypt. Electronic address: shimaa_khaled95@yahoo.com.
  • Makled MN; Department of Pharmacology and Toxicology, Faculty of Pharmacy, Mansoura University, Mansoura, Egypt.
  • Nader MA; Department of Pharmacology and Toxicology, Faculty of Pharmacy, Mansoura University, Mansoura, Egypt.
Life Sci ; 260: 118426, 2020 Nov 01.
Article en En | MEDLINE | ID: mdl-32937159
AIMS: Tobacco smoking is a major health problem associated with lung and liver damage. Lung and liver damage secondary to tobacco smoking is mediated through nicotine-induced oxidative stress. Therefore, we hypothesized that antioxidant treatment with tiron may improve nicotine-induced lung and liver damage. MATERIALS AND METHODS: Rats were divided into six groups, a control, nicotine (10 mg/kg/day, i.p.; for 8 weeks) and tiron (100 or 200 mg/kg/day, i.p.; for 8 weeks) with or without nicotine administration. KEY FINDINGS: Tiron improved survival rate and attenuated lung and liver damage as reflected by decreased total and differential cell counts, lactate dehydrogenase (LDH) activity in bronchoalveolar lavage fluid (BALF) and decreased alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP) in serum; also histopathological examination confirmed the protective effect of tiron in lung and liver tissues of nicotine treated rats. Tiron attenuated dyslipidemia, which is associated with nicotine. These ameliorative effects of tiron may be mainly due to its antioxidant effect as proved by a significant decrease in malondialdehyde (MDA) content, reactive oxygen species (ROS) and total nitrite/nitrate (NOx) levels, and increase in reduced glutathione (GSH) level, catalase (CAT) and superoxide dismutase (SOD) activities. This is likely related to suppression of protein levels of NADPH oxidase enzyme (NOX1), inducible nitric oxide synthase (iNOS), nuclear factor kappa B (NF-κB) and tumor necrosis factor alpha (TNF-α); and up-regulation of protein levels of nuclear factor erythroid-2 (Nrf2). SIGNIFICANCE: This makes tiron (synthetic analogue of vitamin E) good candidate for future use to minimize nicotine's hazards among smokers.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Sal Disódica del Ácido 1,2-Dihidroxibenceno-3,5-Disulfónico / Lesión Pulmonar / Enfermedad Hepática Inducida por Sustancias y Drogas / Nicotina / Antioxidantes Tipo de estudio: Etiology_studies Límite: Animals Idioma: En Revista: Life Sci Año: 2020 Tipo del documento: Article Pais de publicación: Países Bajos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Sal Disódica del Ácido 1,2-Dihidroxibenceno-3,5-Disulfónico / Lesión Pulmonar / Enfermedad Hepática Inducida por Sustancias y Drogas / Nicotina / Antioxidantes Tipo de estudio: Etiology_studies Límite: Animals Idioma: En Revista: Life Sci Año: 2020 Tipo del documento: Article Pais de publicación: Países Bajos