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Effects of Dimethyl Sulfoxide on the Pluripotency and Differentiation Capacity of Mouse Embryonic Stem Cells.
Yi, Jun-Koo; Park, Song; Ha, Jae-Jung; Kim, Dae-Hyun; Huang, Hai; Park, Si-Jun; Lee, Mee-Hyun; Ryoo, Zae-Young; Kim, Sung-Hyun; Kim, Myoung-Ok.
Afiliación
  • Yi JK; Department of Embryo Transfer Research, Gyeongbuk Livestock Research Institute, Yeongju, Korea.
  • Park S; Core Protein Resources Center, DGIST, Daegu, Korea.
  • Ha JJ; Department of Embryo Transfer Research, Gyeongbuk Livestock Research Institute, Yeongju, Korea.
  • Kim DH; Department of Embryo Transfer Research, Gyeongbuk Livestock Research Institute, Yeongju, Korea.
  • Huang H; Department of Life Sciences and Biotechnology, School of Life Sciences and Biotechnology, Kyungpook National University, Daegu, Korea.
  • Park SJ; Department of Biomedical Science, College of Korean Medicine, Dongshin University, Naju, Korea.
  • Lee MH; Department of Life Science Analysis, Korea Polytechnics University, Nonsan, Korea.
  • Ryoo ZY; Department of Biomedical Science, College of Korean Medicine, Dongshin University, Naju, Korea.
  • Kim SH; Department of Animal Science and Biotechnology, School of Animal BT Sciences Kyungpook National University, Sangju, Korea.
  • Kim MO; Department of Life Sciences and Biotechnology, School of Life Sciences and Biotechnology, Kyungpook National University, Daegu, Korea.
Cell Reprogram ; 22(5): 244-253, 2020 10.
Article en En | MEDLINE | ID: mdl-32936029
Mouse embryonic stem cells (mESCs) go through self-renewal in the existence of the cytokine leukemia inhibitory factor (LIF). LIF is added to the mouse stem cells culture medium, and its removal results in fast differentiation. Dimethyl sulfoxide (DMSO) is one of the most used solvents in drug test. We exposed 4-day mESC cultures to different concentrations of DMSO (0.1%, 0.5%, 1.0%, and 2.0%) to identify the safest dose exhibiting efficacy as a solvent. mESCs grown under general pluripotency conditions in the absence of LIF were treated with DMSO. In addition, as a control for differentiation, mESCs were grown in the absence of LIF. DMSO upregulated the mRNA expression level of pluripotency markers. Moreover, DMSO reduced the mRNA expression levels of ectodermal marker (ß-tubulin3), mesodermal marker (Hand1), and endodermal markers (Foxa2 and Sox17) in mESCs. These results indicate that DMSO treatment enhances the pluripotency and disrupts the differentiation of mESCs. We also show that members of the Tet oncogene family are critical to inhibiting the differentiation and methylation of mESCs. DMSO is appropriate to sustain the pluripotency of mESCs in the absence of LIF, and that mESCs can be sustained in an undifferentiated state using DMSO. Therefore, DMSO may, in part, function as a substitute for LIF.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Diferenciación Celular / Dimetilsulfóxido / Células Madre Pluripotentes / Factor Inhibidor de Leucemia / Células Madre Embrionarias de Ratones Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Cell Reprogram Año: 2020 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Diferenciación Celular / Dimetilsulfóxido / Células Madre Pluripotentes / Factor Inhibidor de Leucemia / Células Madre Embrionarias de Ratones Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Cell Reprogram Año: 2020 Tipo del documento: Article Pais de publicación: Estados Unidos