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Phytol-Loaded Solid Lipid Nanoparticles as a Novel Anticandidal Nanobiotechnological Approach.
Lima, Tábata L C; Souza, Luanda B F C; Tavares-Pessoa, Lannya C S; Santos-Silva, Alaine M Dos; Cavalcante, Rômulo S; Araújo-Júnior, Raimundo F de; Cornélio, Alianda M; Fernandes-Pedrosa, Matheus F; Chaves, Guilherme Maranhão; Silva-Júnior, Arnóbio Antônio da.
Afiliación
  • Lima TLC; Laboratory of Pharmaceutical Technology and Biotechnology, Department of Pharmacy, Federal University of Rio Grande do Norte (UFRN), Gal. Gustavo Cordeiro de Farias, S/N, Petrópolis, Natal-RN 59072-570, Brazil.
  • Souza LBFC; Department of Clinical and Toxicological Analyses, Federal University of Rio Grande do Norte (UFRN), Gal, Gustavo Cordeiro de Faria, S/N, Petrópolis, Natal-RN 59084-100, Brazil.
  • Tavares-Pessoa LCS; Laboratory of Pharmaceutical Technology and Biotechnology, Department of Pharmacy, Federal University of Rio Grande do Norte (UFRN), Gal. Gustavo Cordeiro de Farias, S/N, Petrópolis, Natal-RN 59072-570, Brazil.
  • Santos-Silva AMD; Laboratory of Pharmaceutical Technology and Biotechnology, Department of Pharmacy, Federal University of Rio Grande do Norte (UFRN), Gal. Gustavo Cordeiro de Farias, S/N, Petrópolis, Natal-RN 59072-570, Brazil.
  • Cavalcante RS; Department of Morphology, Federal University of Rio Grande do Norte (UFRN), Natal-RN 59.078-970, Brazil.
  • Araújo-Júnior RF; Department of Morphology, Federal University of Rio Grande do Norte (UFRN), Natal-RN 59.078-970, Brazil.
  • Cornélio AM; Department of Morphology, Federal University of Rio Grande do Norte (UFRN), Natal-RN 59.078-970, Brazil.
  • Fernandes-Pedrosa MF; Laboratory of Pharmaceutical Technology and Biotechnology, Department of Pharmacy, Federal University of Rio Grande do Norte (UFRN), Gal. Gustavo Cordeiro de Farias, S/N, Petrópolis, Natal-RN 59072-570, Brazil.
  • Chaves GM; Department of Clinical and Toxicological Analyses, Federal University of Rio Grande do Norte (UFRN), Gal, Gustavo Cordeiro de Faria, S/N, Petrópolis, Natal-RN 59084-100, Brazil.
  • Silva-Júnior AAD; Laboratory of Pharmaceutical Technology and Biotechnology, Department of Pharmacy, Federal University of Rio Grande do Norte (UFRN), Gal. Gustavo Cordeiro de Farias, S/N, Petrópolis, Natal-RN 59072-570, Brazil.
Pharmaceutics ; 12(9)2020 Sep 13.
Article en En | MEDLINE | ID: mdl-32933144
Phytol is a diterpene alcohol and can be found as a product of the metabolism of chlorophyll in plants. This compound has been explored as a potential antimicrobial agent, but it is insoluble in water. In this study, we describe a novel approach for an interesting anticandidal drug delivery system containing phytol. Different formulations of phytol-loaded solid lipid nanoparticles (SLN) were designed and tested using a natural lipid, 1,3-distearyl-2-oleyl-glycerol (TG1). Different compositions were considered to obtain three formulations with 1:10, 1:5, and 1:3 w/w phytol/TG1 ratios. All the formulations were prepared by emulsification solvent evaporation method and had their physicochemical properties assessed. The biocompatibility assay was performed in the HEK-293 cell line and the antifungal efficacy was demonstrated in different strains of Candida ssp., including different clinical isolates. Spherical and uniform SLN (<300 nm, PdI < 0.2) with phytol-loading efficiency >65% were achieved. Phytol-loaded SLN showed a dose-dependent cytotoxic effect in the HEK-293 cell line. The three tested formulations of phytol-loaded SLN considerably enhanced the minimal inhibitory concentration of phytol against 15 strains of Candida spp. Considering the clinical isolates, the formulations containing the highest phytol/TG1 ratios showed MICs at 100%. Thus, the feasibility and potential of phytol-loaded SLN was demonstrated in vitro, being a promising nanocarrier for phytol delivery from an anticandidal approach.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Pharmaceutics Año: 2020 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Pharmaceutics Año: 2020 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Suiza