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Simultaneous Visualization of Wet Cells and Nanostructured Biomaterials in SEM using Ionic Liquids.
Lee, Bryan E J; DiCecco, Liza-Anastasia; Exir, Hourieh; Weck, Arnaud; Sask, Kyla N; Grandfield, Kathryn.
Afiliación
  • Lee BEJ; School of Biomedical Engineering, McMaster University, 1280 Main Street West, Hamilton, ON, L8S 4L7, Canada.
  • DiCecco LA; Department of Materials Science and Engineering, McMaster University, 1280 Main Street West, Hamilton, ON, L8S 4L7, Canada.
  • Exir H; Department of Physics, University of Ottawa, 150 Louis-Pasteur Pvt, Ottawa, ON, K1N 6N5, Canada.
  • Weck A; Centre for Research in Photonics, University of Ottawa, 25 Templeton Street, Ottawa, ON, K1N 6N5, Canada.
  • Sask KN; Department of Physics, University of Ottawa, 150 Louis-Pasteur Pvt, Ottawa, ON, K1N 6N5, Canada.
  • Grandfield K; Centre for Research in Photonics, University of Ottawa, 25 Templeton Street, Ottawa, ON, K1N 6N5, Canada.
Chembiochem ; 22(3): 571-576, 2021 02 02.
Article en En | MEDLINE | ID: mdl-32918376
This work presents a successful methodology to image mammalian cells adhered to nanostructured titanium by using scanning electron microscopy (SEM) operating in low-vacuum mode following ionic liquid treatment. Human osteoblast-like Saos-2 cells were treated with a room-temperature ionic liquid, 1-ethyl-3-methylimidazolium tetrafluoroborate, and subsequently imaged on titanium by SEM. Titanium substrates were modified to create laser-induced periodic surface structures (LIPSS) for visualization at the submicron scale. By using a combination of fluorescence-based cell metabolism along with light microscopy and SEM image analysis, the shape and location of irradiated cells were confirmed to be unchanged after multiple irradiation sessions; the viability of minimally irradiated cells was also unaltered. The wet imaging conditions combined with a rapid facile protocol using ionic liquid allows this technique to fulfill a niche in examining cellular behavior on biomaterials with submicron surface features. The demonstrated method to track observed cell adhesion to submicron surface features by SEM has great implications for understanding cell migration on nanostructured surfaces as well as the exploration of simpler SEM preparation methods for cellular imaging.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Materiales Biocompatibles / Nanoestructuras / Líquidos Iónicos Tipo de estudio: Guideline Límite: Humans Idioma: En Revista: Chembiochem Asunto de la revista: BIOQUIMICA Año: 2021 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Alemania
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Materiales Biocompatibles / Nanoestructuras / Líquidos Iónicos Tipo de estudio: Guideline Límite: Humans Idioma: En Revista: Chembiochem Asunto de la revista: BIOQUIMICA Año: 2021 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Alemania