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4-Aminobiphenyl suppresses homologous recombination repair by a reactive oxygen species-dependent p53/miR-513a-5p/p53 loop.
Lin, Heng-Dao; Yao, Chao-Ling; Ou, Wei-Jen; Luo, Yueh-Hsia; Chen, Ssu Ching.
Afiliación
  • Lin HD; Department of Life Sciences, National Central University, Jhongli, Taiwan.
  • Yao CL; Department of Chemical Engineering and Materials Science, Yuan Ze University, No. 135 Yuan-Tung Road Taoyuan City 32003, Taiwan.
  • Ou WJ; Hematology-Oncology Section, LANDSEED Hospital, Jhongli, Taiwan.
  • Luo YH; Department of Life Sciences, National Central University, Jhongli, Taiwan.
  • Chen SC; Department of Life Sciences, National Central University, Jhongli, Taiwan. Electronic address: osycchna@ksts.seed.net.tw.
Toxicology ; 444: 152580, 2020 11.
Article en En | MEDLINE | ID: mdl-32905825
4-Aminobiphenyl (4-ABP), a well-known human carcinogen, can cause oxidative DNA damage and induce miR-513a-5p. However, the interplay between miR-513a-5p and DNA damage remains unclear. In our result of ChIP assay, we speculated that p53 as transcription factor could regulate miR-513a-5p expression. In addition, we found that miR-513a-5p-induced by 4-ABP could suppress p53 expression and HR repair activity. On the other hand, the levels of p53, miR-513a-5p, and γH2AX were attenuated by 5 mM N-acetyl-l-cysteine (NAC) pretreatment, indicating that the reactive oxygen species (ROS)-dependent p53-miR-513a-5p was involved in DSB repair in 4-ABP-treated cells. These findings indicated that the ROS/p53/miR-513a-5p/p53 loop axis plays a relevant role in regulating HR repair which may facilitate our understanding of molecular mechanisms regarding how miR-513a-5p impacts DSB repair in 4-ABP-treated cells.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Carcinógenos / Proteína p53 Supresora de Tumor / MicroARNs / Reparación del ADN por Recombinación / Compuestos de Aminobifenilo Límite: Humans Idioma: En Revista: Toxicology Año: 2020 Tipo del documento: Article País de afiliación: Taiwán Pais de publicación: Irlanda
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Carcinógenos / Proteína p53 Supresora de Tumor / MicroARNs / Reparación del ADN por Recombinación / Compuestos de Aminobifenilo Límite: Humans Idioma: En Revista: Toxicology Año: 2020 Tipo del documento: Article País de afiliación: Taiwán Pais de publicación: Irlanda