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Spoligotyping of Mycobacterium tuberculosis isolates using Luminex®-based method in Lebanon.
Masoud, Khaldoun; Araj, George F; Reslan, Lina; Fadlallah, Sukayna; Wehbe, Michel; Itani, Lina; Avedissian, Aline; Dbaibo, Ghassan; Saade, Antoine; Refrégier, Guislaine; Sola, Christophe; Matar, Ghassan M.
Afiliación
  • Masoud K; WHO Collaborating Center for Reference and Research on Bacterial Pathogens, Center for Infectious Diseases Research (CIDR), Faculty of Medicine, American University of Beirut, Beirut, Lebanon. khaldoun.masoud@gmail.com.
  • Araj GF; WHO Collaborating Center for Reference and Research on Bacterial Pathogens, Center for Infectious Diseases Research (CIDR), Faculty of Medicine, American University of Beirut, Beirut, Lebanon. garaj@aub.edu.lb.
  • Reslan L; WHO Collaborating Center for Reference and Research on Bacterial Pathogens, Center for Infectious Diseases Research (CIDR), Faculty of Medicine, American University of Beirut, Beirut, Lebanon. lr13@aub.edu.lb.
  • Fadlallah S; WHO Collaborating Center for Reference and Research on Bacterial Pathogens, Center for Infectious Diseases Research (CIDR), Faculty of Medicine, American University of Beirut, Beirut, Lebanon. smf28@mail.aub.edu.
  • Wehbe M; WHO Collaborating Center for Reference and Research on Bacterial Pathogens, Center for Infectious Diseases Research (CIDR), Faculty of Medicine, American University of Beirut, Beirut, Lebanon. Michel-wehbe@hotmail.com.
  • Itani L; WHO Collaborating Center for Reference and Research on Bacterial Pathogens, Center for Infectious Diseases Research (CIDR), Faculty of Medicine, American University of Beirut, Beirut, Lebanon. li01@aub.edu.lb.
  • Avedissian A; WHO Collaborating Center for Reference and Research on Bacterial Pathogens, Center for Infectious Diseases Research (CIDR), Faculty of Medicine, American University of Beirut, Beirut, Lebanon. aa75@aub.edu.lb.
  • Dbaibo G; WHO Collaborating Center for Reference and Research on Bacterial Pathogens, Center for Infectious Diseases Research (CIDR), Faculty of Medicine, American University of Beirut, Beirut, Lebanon. gdbaibo@aub.edu.lb.
  • Saade A; Ministry of Public Health, Beirut, Lebanon.
  • Refrégier G; Institute for Integrative Biology of the Cell, University Paris-Sud, University Paris-Saclay, France. guislaine.refregier@u-psud.fr.
  • Sola C; Institute for Integrative Biology of the Cell, University Paris-Sud, University Paris-Saclay, France. christophe.sola@u-psud.fr.
  • Matar GM; WHO Collaborating Center for Reference and Research on Bacterial Pathogens, Center for Infectious Diseases Research (CIDR), Faculty of Medicine, American University of Beirut, Beirut, Lebanon. gmatar@aub.edu.lb.
J Infect Dev Ctries ; 14(8): 878-885, 2020 08 31.
Article en En | MEDLINE | ID: mdl-32903232
INTRODUCTION: Data about the genotypes of circulating Mycobacterium tuberculosis isolates (MTB) in Lebanon are scarce. This study was undertaken to reveal the spoligotypes of MTB isolates recovered from patients in Lebanon. METHODOLOGY: MTB isolates from 49 patients living in Lebanon were recovered and identified. The samples were heat killed and subjected to DNA extraction. Spoligotyping was performed using microbeads from TB-SPOL Kit and the fluorescence intensity was measured using Luminex 200®. Generated patterns were assigned to families using the SITVIT2 international database of the Pasteur Institute of Guadeloupe and compared. RESULTS: The spoligotyping of the 49 MTB isolates revealed that 31 isolates belonged to Lineage 4 (Euro-American, 63.3%), 12 to Lineage 3 (East- African Indian, 24.5%), 3 to Lineage 2 (East Asian, 6%) and 2 were unknown. Over half of the genotypes (16 of 30) harbored SIT127 supposed to belong to the L4.5 sublineage. One isolate belonging to the rare Manu-Ancestor SIT523 was recovered for the first time in Lebanon, being associated with highly virulent extensively drug-resistant (XDR) MTB phenotype. CONCLUSION: The application of the Spoligotyping Multiplex Luminex® method is an efficient, discriminatory and rapid method to use for first-lane genotyping of MTB isolates. Though humble numbers were tested, this study is one of the first to describe the genomic diversity and epidemiology of MTB isolates of Lebanon, and suggests an increasing prevalence of SIT127 in the country.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Tuberculosis / Técnicas de Tipificación Bacteriana / Mycobacterium tuberculosis Tipo de estudio: Risk_factors_studies Límite: Humans País/Región como asunto: Asia Idioma: En Revista: J Infect Dev Ctries Asunto de la revista: DOENCAS TRANSMISSIVEIS Año: 2020 Tipo del documento: Article País de afiliación: Líbano Pais de publicación: Italia
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Tuberculosis / Técnicas de Tipificación Bacteriana / Mycobacterium tuberculosis Tipo de estudio: Risk_factors_studies Límite: Humans País/Región como asunto: Asia Idioma: En Revista: J Infect Dev Ctries Asunto de la revista: DOENCAS TRANSMISSIVEIS Año: 2020 Tipo del documento: Article País de afiliación: Líbano Pais de publicación: Italia