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Mineral and Amino Acid Profiling of Different Hematopoietic Populations from the Mouse Bone Marrow.
Girotra, Mukul; Monnard, Caroline; Konz, Tobias; Sizzano, Federico; Goulet, Laurence; Godin, Jean-Philippe; Coukos, George; Rezzi, Serge; Vannini, Nicola.
Afiliación
  • Girotra M; Department of Oncology, Ludwig Cancer Institute, University of Lausanne, 1066 Epalinges, Switzerland.
  • Monnard C; Nestlé Research, EPFL Innovation Park, 1015 Lausanne, Switzerland.
  • Konz T; Nestlé Research, EPFL Innovation Park, 1015 Lausanne, Switzerland.
  • Sizzano F; Nestlé Research, EPFL Innovation Park, 1015 Lausanne, Switzerland.
  • Goulet L; Nestlé Research, EPFL Innovation Park, 1015 Lausanne, Switzerland.
  • Godin JP; Nestlé Research, Vers-chez-les-Blanc, 1000 Lausanne, Switzerland.
  • Coukos G; Department of Oncology, Ludwig Cancer Institute, University of Lausanne, 1066 Epalinges, Switzerland.
  • Rezzi S; Nestlé Research, EPFL Innovation Park, 1015 Lausanne, Switzerland.
  • Vannini N; Department of Oncology, Ludwig Cancer Institute, University of Lausanne, 1066 Epalinges, Switzerland.
Int J Mol Sci ; 21(17)2020 Sep 03.
Article en En | MEDLINE | ID: mdl-32899421
Steady hematopoiesis is essential for lifelong production of all mature blood cells. Hematopoietic stem and progenitor cells (HSPCs) found in the bone marrow ensure hematopoietic homeostasis in an organism. Failure of this complex process, which involves a fine balance of self-renewal and differentiation fates, often result in severe hematological conditions such as leukemia and lymphoma. Several molecular and metabolic programs, internal or in close interaction with the bone marrow niche, have been identified as important regulators of HSPC function. More recently, nutrient sensing pathways have emerged as important modulators of HSC homing, dormancy, and function in the bone marrow. Here we describe a method for reliable measurement of various amino acids and minerals in different rare bone marrow (BM) populations, namely HSPCs. We found that the amino acid profile of the most primitive hematopoietic compartments (KLS) did not differ significantly from the one of their direct progenies (common myeloid progenitor CMP), while granulocyte-monocyte progenitors (GMPs), on the opposite of megakaryocyte-erythroid progenitors (MEPs), have higher content of the majority of amino acids analyzed. Additionally, we identified intermediates of the urea cycle to be differentially expressed in the KLS population and were found to lower mitochondrial membrane potential, an established readout on self-renewal capability. Moreover, we were able to profile for the first time 12 different minerals and detect differences in elemental contents between different HSPC compartments. Importantly, essential dietary trace elements, such as iron and molybdenum, were found to be enriched in granulocyte-monocyte progenitors (GMPs). We envision this amino acid and mineral profiling will allow identification of novel metabolic and nutrient sensing pathways important in HSPC fate regulation.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Médula Ósea / Células Madre Hematopoyéticas / Aminoácidos / Hematopoyesis / Minerales Límite: Animals Idioma: En Revista: Int J Mol Sci Año: 2020 Tipo del documento: Article País de afiliación: Suiza Pais de publicación: Suiza
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Médula Ósea / Células Madre Hematopoyéticas / Aminoácidos / Hematopoyesis / Minerales Límite: Animals Idioma: En Revista: Int J Mol Sci Año: 2020 Tipo del documento: Article País de afiliación: Suiza Pais de publicación: Suiza