Association of PRKCA expression and polymorphisms with layer duck eggshell quality.

Tan, G H; Li, J Z; Zhang, Y Y; You, M F; Liao, C M; Zhang, Y G

Tan, G H; Li, J Z; Zhang, Y Y; You, M F; Liao, C M; Zhang, Y G.

Afiliación

Tan GH; Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Ministry of Education, College of Animal Science, Guizhou University , Guiyang, Guizhou, People's Republic of China.
Li JZ; Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Ministry of Education, College of Animal Science, Guizhou University , Guiyang, Guizhou, People's Republic of China.
Zhang YY; Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Ministry of Education, College of Animal Science, Guizhou University , Guiyang, Guizhou, People's Republic of China.
You MF; Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Ministry of Education, College of Animal Science, Guizhou University , Guiyang, Guizhou, People's Republic of China.
Liao CM; Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Ministry of Education, College of Animal Science, Guizhou University , Guiyang, Guizhou, People's Republic of China.
Zhang YG; Tiantang Town Agricultural Technology Management Station , Tongren City, People's Republic of China.

Br Poult Sci ; 62(1): 8-16, 2021 Feb.

Article en En | MEDLINE | ID: mdl-32893664

RESUMEN

1. Eggshell quality is important for the poultry industry. Calcium is deposited during eggshell formation, and protein kinase C alpha (PRKCA) is involved in transmembrane transport of calcium ions in cells. However, the biological function of PRKCA in poultry is still not understood. Therefore, the aim of this study was to explore the association of mRNA expression and single nucleotide polymorphisms (SNPs) of the PRKCA gene with eggshell quality in laying ducks. 2. The mRNA expression and SNPs of the PRKCA gene were detected by real-time fluorescence quantitative PCR (qRT-PCR) and sequencing of PCR products in 45-week-old female Sansui ducks, which is a high production layer duck breed in China. The association of mRNA expression and SNPs in the PRKCA gene with layer duck eggshell traits was analysed using SPSS (v18.0) software. 3. The results demonstrated that PRKCA mRNA was widely expressed in all examined tissues, and expression was highest in kidney and lowest in the gizzard. Furthermore, the PRKCA mRNA level in uterus was significantly positively correlated with eggshell strength and eggshell weight (P < 0.05). Three novel SNPs, the synonymous mutations of g.9571770 T > C in exon 5, g.9583222 C > T and g.9583227 G > A in exon 7, were found in the PRKCA gene, giving four haplotypes and 10 diplotypes, which affected the mRNA secondary structure and free energy. The g.9583222 C > T and g.9583227 G > A mutations were significantly associated with eggshell strength (P < 0.05). Diplotype H1H1 was advantageous for increasing the strength and thickness of an eggshell. 4. In conclusion, the study showed that the mRNA transcription and genetic variation in the PRKCA gene could significantly affect the strength of duck eggshell and that the PRKCA gene is an important candidate gene for improving eggshell quality in poultry.

Asunto(s)

Patos; Cáscara de Huevo; Animales; Pollos/genética; China; Patos/genética; Femenino; Polimorfismo de Nucleótido Simple; Proteína Quinasa C-alfa

Palabras clave

PRKCA gene; Laying duck; SNPs; eggshell quality; gene expression

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Patos / Cáscara de Huevo Tipo de estudio: Risk_factors_studies Límite: Animals País/Región como asunto: Asia Idioma: En Revista: Br Poult Sci Año: 2021 Tipo del documento: Article Pais de publicación: Reino Unido

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