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High-throughput microRNA profile in adult and pediatric primary glioblastomas: the role of miR-10b-5p and miR-630 in the tumor aggressiveness.
Junior, Luiz Guilherme Darrigo; Baroni, Mirella; Lira, Régia Caroline Peixoto; Teixeira, Silvia; Fedatto, Paola Fernanda; Silveira, Vanessa Silva; Suazo, Veridiana Kill; Veronez, Luciana Chain; Panepucci, Rodrigo Alexandre; Antônio, David Santos Marco; Yunes, José Andres; Brandalise, Silvia Regina; Dos Santos Aguiar, Simone; Neder, Luciano; de Oliveira, Ricardo Santos; Machado, Hélio Rubens; Carlotti, Carlos Gilberto; Tone, Luiz Gonzaga; Valera, Elvis Terci; Scrideli, Carlos Alberto.
Afiliación
  • Junior LGD; Department of Pediatrics, Ribeirao Preto Medical School, University of Sao Paulo - USP, Avenida dos Bandeirantes, 3900, Ribeirao Preto, 14049-900, Brazil.
  • Baroni M; Department of Genetics, Ribeirão Preto Medical School, University of Sao Paulo, Ribeirão Preto, SP, Brazil.
  • Lira RCP; Department of Pediatrics, Ribeirao Preto Medical School, University of Sao Paulo - USP, Avenida dos Bandeirantes, 3900, Ribeirao Preto, 14049-900, Brazil.
  • Teixeira S; Centro Universitário CESMAC, Maceió, AL, Brazil.
  • Fedatto PF; Department of Pediatrics, Ribeirao Preto Medical School, University of Sao Paulo - USP, Avenida dos Bandeirantes, 3900, Ribeirao Preto, 14049-900, Brazil.
  • Silveira VS; Department of Pediatrics, Ribeirao Preto Medical School, University of Sao Paulo - USP, Avenida dos Bandeirantes, 3900, Ribeirao Preto, 14049-900, Brazil.
  • Suazo VK; Department of Genetics, Ribeirão Preto Medical School, University of Sao Paulo, Ribeirão Preto, SP, Brazil.
  • Veronez LC; Department of Pediatrics, Ribeirao Preto Medical School, University of Sao Paulo - USP, Avenida dos Bandeirantes, 3900, Ribeirao Preto, 14049-900, Brazil.
  • Panepucci RA; Department of Pediatrics, Ribeirao Preto Medical School, University of Sao Paulo - USP, Avenida dos Bandeirantes, 3900, Ribeirao Preto, 14049-900, Brazil.
  • Antônio DSM; Hemocentro Foundation, Ribeirão Preto Medical School, University of Sao Paulo, Ribeirão Preto, SP, Brazil.
  • Yunes JA; Department of Research and Development, Fleury Group, São Paulo, SP, Brazil.
  • Brandalise SR; Boldrini's Children Center, Campinas, SP, Brazil.
  • Dos Santos Aguiar S; State University of Campinas, Campinas, SP, Brazil.
  • Neder L; Boldrini's Children Center, Campinas, SP, Brazil.
  • de Oliveira RS; State University of Campinas, Campinas, SP, Brazil.
  • Machado HR; Department of Pathology, Ribeirão Preto Medical School, University of Sao Paulo, Ribeirão Preto, SP, Brazil.
  • Carlotti CG; Division of Neurosurgery, Department of Surgery, Ribeirão Preto Medical School, University of Sao Paulo, Ribeirão Preto, SP, Brazil.
  • Tone LG; Division of Neurosurgery, Department of Surgery, Ribeirão Preto Medical School, University of Sao Paulo, Ribeirão Preto, SP, Brazil.
  • Valera ET; Division of Neurosurgery, Department of Surgery, Ribeirão Preto Medical School, University of Sao Paulo, Ribeirão Preto, SP, Brazil.
  • Scrideli CA; Department of Pediatrics, Ribeirao Preto Medical School, University of Sao Paulo - USP, Avenida dos Bandeirantes, 3900, Ribeirao Preto, 14049-900, Brazil.
Mol Biol Rep ; 47(9): 6949-6959, 2020 Sep.
Article en En | MEDLINE | ID: mdl-32888124
Glioblastoma (GBM) is the most common primary malignant neoplasm of the central nervous system and, despite the standard therapy; the patients' prognoses remain dismal. The miRNA expression profiles have been associated with patient prognosis, suggesting that they may be helpful for tumor diagnosis and classification as well as predictive of tumor response to treatment. We described the microRNA expression profile of 29 primary GBM samples (9 pediatric GBMs) and 11 non-neoplastic white matter samples as controls (WM) by microarray analysis and we performed functional in vitro assays on these 2 most differentially expressed miRNAs. Hierarchical clustering analysis showed 3 distinct miRNA profiles, two of them in the GBM samples and a group consisting only of cerebral white matter. When adult and pediatric GBMs were compared to WM, 37 human miRNAs were found to be differentially expressed, with miR-10b-5p being the most overexpressed and miR-630 the most underexpressed. The overexpression of miR-630 was associated with reduced cell proliferation and invasion in the U87 GBM cell line, whereas the inhibition of miR-10b-5p reduced cell proliferation and colony formation in the U251 GBM cell line, suggesting that these miRNAs may act as tumor-suppressive and oncogenic miRNAs, respectively. The present study highlights the distinct epigenetic profiling of adult and pediatric GBMs and underscores the biological importance of mir-10b-5p and miR-630 for the pathobiology of these lethal tumors.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: ARN Neoplásico / Regulación Neoplásica de la Expresión Génica / Glioblastoma / Perfilación de la Expresión Génica / MicroARNs Límite: Adolescent / Adult / Aged / Child / Child, preschool / Female / Humans / Male / Middle aged Idioma: En Revista: Mol Biol Rep Año: 2020 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: ARN Neoplásico / Regulación Neoplásica de la Expresión Génica / Glioblastoma / Perfilación de la Expresión Génica / MicroARNs Límite: Adolescent / Adult / Aged / Child / Child, preschool / Female / Humans / Male / Middle aged Idioma: En Revista: Mol Biol Rep Año: 2020 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Países Bajos