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The tight junction protein cingulin regulates the vascular response to burn injury in a mouse model.
Zhuravleva, Kristina; Goertz, Ole; Wölkart, Gerald; Guillemot, Laurent; Petzelbauer, Peter; Lehnhardt, Marcus; Schmidt, Kurt; Citi, Sandra; Schossleitner, Klaudia.
Afiliación
  • Zhuravleva K; Department of Plastic and Hand Surgery, Burn Center, BG-University Hospital Bergmannsheil, Ruhr University Bochum, Bochum, Germany; Department of Plastic, Reconstructive and Aesthetic Surgery, Hand Surgery, Martin-Luther Hospital, Berlin, Germany.
  • Goertz O; Department of Plastic and Hand Surgery, Burn Center, BG-University Hospital Bergmannsheil, Ruhr University Bochum, Bochum, Germany; Department of Plastic, Reconstructive and Aesthetic Surgery, Hand Surgery, Martin-Luther Hospital, Berlin, Germany.
  • Wölkart G; Department of Pharmacology and Toxicology, Institute of Pharmaceutical Sciences, Karl-Franzens-Universität Graz, Graz, Austria.
  • Guillemot L; Department of Cell Biology, Faculty of Sciences, and Institute of Genetics and Genomics of Geneva, University of Geneva, Switzerland.
  • Petzelbauer P; Skin and Endothelium Research Division, Department of Dermatology, Medical University Vienna, Vienna, Austria.
  • Lehnhardt M; Department of Plastic and Hand Surgery, Burn Center, BG-University Hospital Bergmannsheil, Ruhr University Bochum, Bochum, Germany.
  • Schmidt K; Department of Pharmacology and Toxicology, Institute of Pharmaceutical Sciences, Karl-Franzens-Universität Graz, Graz, Austria.
  • Citi S; Department of Cell Biology, Faculty of Sciences, and Institute of Genetics and Genomics of Geneva, University of Geneva, Switzerland.
  • Schossleitner K; Skin and Endothelium Research Division, Department of Dermatology, Medical University Vienna, Vienna, Austria. Electronic address: klaudia.schossleitner@muv.ac.at.
Microvasc Res ; 132: 104067, 2020 11.
Article en En | MEDLINE | ID: mdl-32877697
Edema formation due to the collapse of physiological barriers and the associated delayed healing process is still a central problem in the treatment of burn injuries. In healthy individuals, tight junctions form a barrier to fluid and small molecules. Cingulin is a cytoplasmic component of tight junctions and is involved in the regulation of the paracellular barrier. Endothelial specific cingulin knock-out mice provide new insight into the influence of tight junction proteins on edema formation and angiogenesis during wound healing. Knock-out mice lacking the head domain of cingulin in endothelial cells (CgnΔEC) were created by breeding Cgnfl/fl mice with Tie1-cre mice. Using a no-touch hot air jet a burn trauma was induced on the ear of the mouse. Over a period of 12 days microcirculatory parameters such as edema formation, angiogenesis and leukocyte-endothelial interactions were visualized using intravital fluorescence microscopy. At baseline, CgnΔEC mice surprisingly showed significantly less tracer extravasation compared to Cgnfl/fl littermates, whereas, after burn injury, edema was consistently higher in CgnΔEC mice. Non-perfused area after wounding was increased, but there was no difference in vessel diameters, contraction or dilation of arteries in CgnΔEC mice. Moreover, cingulin knock-out did not cause a difference in leukocyte adhesion after burn injury. In summary, cingulin limits non-perfused area after burn injury and maintains the paracellular barrier of blood vessels. Since edema formation with serious systemic effects is a central problem of burn wounds, understanding the importance of tight junction proteins might help to find new treatment strategies for burn wounds.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Piel / Cicatrización de Heridas / Quemaduras / Uniones Estrechas / Células Endoteliales / Edema / Microvasos / Proteínas de la Membrana Límite: Animals Idioma: En Revista: Microvasc Res Año: 2020 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Piel / Cicatrización de Heridas / Quemaduras / Uniones Estrechas / Células Endoteliales / Edema / Microvasos / Proteínas de la Membrana Límite: Animals Idioma: En Revista: Microvasc Res Año: 2020 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Estados Unidos