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A High-Throughput Assay for Circulating Antibodies Directed Against the S Protein of Severe Acute Respiratory Syndrome Coronavirus 2.
Weiss, Svenja; Klingler, Jéromine; Hioe, Catarina; Amanat, Fatima; Baine, Ian; Arinsburg, Suzanne; Kojic, Erna Milunka; Stoever, Jonathan; Liu, Sean T H; Jurczyszak, Denise; Bermudez-Gonzalez, Maria; Simon, Viviana; Krammer, Florian; Zolla-Pazner, Susan.
Afiliación
  • Weiss S; Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, New York, USA.
  • Klingler J; Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, New York, USA.
  • Hioe C; Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, New York, USA.
  • Amanat F; Research Service, James J. Peters Veterans Affairs Medical Center, Bronx, New York, USA.
  • Baine I; Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York, USA.
  • Arinsburg S; Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York, USA.
  • Kojic EM; Graduate School of Biomedical Sciences, Icahn School of Medicine at Mount Sinai, New York, New York, USA.
  • Stoever J; Department of Pathology, Molecular and Cell-Based Medicine, Icahn School of Medicine at Mount Sinai, New York, New York, USA.
  • Liu STH; Department of Pathology, Molecular and Cell-Based Medicine, Icahn School of Medicine at Mount Sinai, New York, New York, USA.
  • Jurczyszak D; Department of Medicine, Mount Sinai West and Morningside, New York, New York, USA.
  • Bermudez-Gonzalez M; Pulmonary and Critical Care Medicine, Mount Sinai West, New York, New York, USA.
  • Simon V; Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York, USA.
  • Krammer F; Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York, USA.
  • Zolla-Pazner S; Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York, USA.
J Infect Dis ; 222(10): 1629-1634, 2020 10 13.
Article en En | MEDLINE | ID: mdl-32860510
More than 24 million infections with the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) were confirmed globally by September 2020. While polymerase chain reaction-based assays are used for diagnosis, there is a need for high-throughput, rapid serologic methods. A Luminex binding assay was developed and used to assess simultaneously the presence of coronavirus disease 2019 (COVID-19)-specific antibodies in human serum and plasma. Clear differentiation was achieved between specimens from infected and uninfected subjects, and a wide range of serum/plasma antibody levels was delineated in infected subjects. All 25 specimens from 18 patients with COVID-19 were positive in the assays with both the trimeric spike and the receptor-binding domain proteins. None of the 13 specimens from uninfected subjects displayed antibodies to either antigen. There was a highly statistically significant difference between the antibody levels of COVID-19-infected and -uninfected specimens (P < .0001). This high-throughput antibody assay is accurate, requires only 2.5 hours, and uses 5 ng of antigen per test.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neumonía Viral / Ensayo de Inmunoadsorción Enzimática / Infecciones por Coronavirus / Técnicas de Laboratorio Clínico / Ensayos Analíticos de Alto Rendimiento / Glicoproteína de la Espiga del Coronavirus / Betacoronavirus / Anticuerpos Antivirales Tipo de estudio: Diagnostic_studies / Observational_studies Límite: Humans Idioma: En Revista: J Infect Dis Año: 2020 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neumonía Viral / Ensayo de Inmunoadsorción Enzimática / Infecciones por Coronavirus / Técnicas de Laboratorio Clínico / Ensayos Analíticos de Alto Rendimiento / Glicoproteína de la Espiga del Coronavirus / Betacoronavirus / Anticuerpos Antivirales Tipo de estudio: Diagnostic_studies / Observational_studies Límite: Humans Idioma: En Revista: J Infect Dis Año: 2020 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos