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FRET-Mediated Observation of Protein-Triggered Conformational Changes in DNA Nanostructures.
Shiu, Simon Chi-Chin; Sakai, Yusuke; Tanner, Julian A; Heddle, Jonathan G.
Afiliación
  • Shiu SC; School of Biomedical Sciences, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong SAR, China.
  • Sakai Y; Malopolska Centre of Biotechnology, Jagiellonian University, Krakow, Poland.
  • Tanner JA; School of Biomedical Sciences, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong SAR, China. jatanner@hku.hk.
  • Heddle JG; Malopolska Centre of Biotechnology, Jagiellonian University, Krakow, Poland. jonathan.heddle@uj.edu.pl.
Methods Mol Biol ; 2208: 69-80, 2021.
Article en En | MEDLINE | ID: mdl-32856256
DNA origami is a powerful technique, which allows virtually limitless 2D or 3D nanostructure designs to be constructed from DNA strands. Such nanostructures can even include programmable nanorobots, which are able to respond to the environment in predetermined ways. DNA aptamers hold particular promise as interfaces, which can enable proteins, peptides, and other non-nucleic acid biomolecules to trigger conformational changes in DNA nanostructures for diagnostic, biosensing, or therapeutic applications. Here, we provide the methodology for FRET-mediated observation of aptamer-triggered conformational change in a DNA origami box nanostructure. The method described can, in principle, be adapted to a wide variety of experimental circumstances where the DNA nanostructure conformational change is mediated by molecular or environmental cues.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: ADN / Proteínas / Transferencia Resonante de Energía de Fluorescencia / Nanoestructuras Idioma: En Revista: Methods Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2021 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: ADN / Proteínas / Transferencia Resonante de Energía de Fluorescencia / Nanoestructuras Idioma: En Revista: Methods Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2021 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos