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A Flow Cytometric Method to Determine Transfection Efficiency.
Mu, Wenli; Homann, Stefanie; Hofmann, Christian; Gorin, Alexandr; Huynh, Diana; Yang, Otto Orlean; Kelesidis, Theodoros.
Afiliación
  • Mu W; David Geffen School of Medicine, University of California, Los Angeles, USA.
  • Homann S; David Geffen School of Medicine, University of California, Los Angeles, USA.
  • Hofmann C; David Geffen School of Medicine, University of California, Los Angeles, USA.
  • Gorin A; David Geffen School of Medicine, University of California, Los Angeles, USA.
  • Huynh D; David Geffen School of Medicine, University of California, Los Angeles, USA.
  • Yang OO; David Geffen School of Medicine, University of California, Los Angeles, USA.
  • Kelesidis T; David Geffen School of Medicine, University of California, Los Angeles, USA.
Bio Protoc ; 9(10)2019 May 20.
Article en En | MEDLINE | ID: mdl-32855997
Mammalian cell transfection is a powerful technique commonly used in molecular biology to express exogenous DNA or RNA in cells and study gene and protein function. Although several transfection strategies have been developed, there is a wide variation with regards to transfection efficiency, cell toxicity and reproducibility. Thus, a sensitive and robust method that can optimize transfection efficiency based not only on expression of the target protein of interest but also on the uptake of the nucleic acids, can be an important tool in molecular biology. Herein, we present a simple, rapid and robust flow cytometric method that can be used as a tool to optimize transfection efficiency while overcoming limitations of prior established methods that quantify transfection efficiency.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Bio Protoc Año: 2019 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
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PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Bio Protoc Año: 2019 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos