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Distance-based quantification of miRNA-21 by the coffee-ring effect using paper devices.
Zhang, Dagan; Wu, Chao; Luan, Chengxin; Gao, Peng; Wang, Huan; Chi, Junjie; Kong, Tiantian.
Afiliación
  • Zhang D; Guangdong Key Laboratory of Biomedical Measurements and Ultrasound Imaging, Department of Biomedical Engineering, Shenzhen University, Shenzhen, 518060, China.
  • Wu C; Suzhou Engineering and Technological Research Center of Natural Medicine and Functional Food, School of Biology and Food Engineering, Suzhou University, Suzhou, 234000, China.
  • Luan C; Department of Hematology, the First Affiliated Hospital of Anhui Medical University, Hefei, 230022, Anhui, China.
  • Gao P; Guangdong Key Laboratory of Biomedical Measurements and Ultrasound Imaging, Department of Biomedical Engineering, Shenzhen University, Shenzhen, 518060, China.
  • Wang H; Guangdong Key Laboratory of Biomedical Measurements and Ultrasound Imaging, Department of Biomedical Engineering, Shenzhen University, Shenzhen, 518060, China. wang_huan@seu.edu.cn.
  • Chi J; Guangdong Key Laboratory of Biomedical Measurements and Ultrasound Imaging, Department of Biomedical Engineering, Shenzhen University, Shenzhen, 518060, China. cjj_2337@163.com.
  • Kong T; Guangdong Key Laboratory of Biomedical Measurements and Ultrasound Imaging, Department of Biomedical Engineering, Shenzhen University, Shenzhen, 518060, China. ttkong@szu.edu.cn.
Mikrochim Acta ; 187(9): 513, 2020 08 24.
Article en En | MEDLINE | ID: mdl-32833099
Enabled by the coffee-ring effect, a paper-based signal transduce method is employed for catalytic hairpin assembly (CHA) amplification and hybridization chain reaction (HCR) to achieve miRNA quantification. Once the target miRNAs appeared, it was circularly used by CHA to initiate HCR amplification to produce a large number of G-quadruplex, which is combined with hemin to form a hemin/G-quadruplex DNAzyme. The DNAzyme catalyzes a colorimetric reaction to produce colored nanoparticles, which were converted to the end edge of the paper by evaporation-driven flow, forming a visible colored band. Higher concentration of miRNA led to more colored nanoparticles and thus a longer colored band that can simply be measured by a ruler. The results of determination of miRNA in samples demonstrate that the relative standard deviation of the proposed approach is 5.2%, highly sensitive and repeatable, with a working range 1.0 to 1000 pM and a LOD of 0.2 pM. The paper-based analytical device as a novel platform offers a new signal transduce pathway toward the detection of low-abundance biomarkers for diagnosis.Graphical abstract Schematic representation of the principle for quantification of miRNA on paper based on the coffee-ring effect.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Papel / Colorimetría / MicroARNs Límite: Humans Idioma: En Revista: Mikrochim Acta Año: 2020 Tipo del documento: Article País de afiliación: China Pais de publicación: Austria
Texto completo
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Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Papel / Colorimetría / MicroARNs Límite: Humans Idioma: En Revista: Mikrochim Acta Año: 2020 Tipo del documento: Article País de afiliación: China Pais de publicación: Austria