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Protein Determination with Molecularly Imprinted Polymer Recognition Combined with Birefringence Liquid Crystal Detection.
Cieplak, Maciej; Weglowski, Rafal; Iskierko, Zofia; Weglowska, Dorota; Sharma, Piyush S; Noworyta, Krzysztof R; D'Souza, Francis; Kutner, Wlodzimierz.
Afiliación
  • Cieplak M; Institute of Physical Chemistry Polish Academy of Sciences, Kasprzaka Str. 44/52, 01-224 Warsaw, Poland.
  • Weglowski R; Faculty of Advanced Technologies and Chemistry, Military University of Technology, Kaliskiego Str. 2, 00-908 Warsaw, Poland.
  • Iskierko Z; Institute of Physical Chemistry Polish Academy of Sciences, Kasprzaka Str. 44/52, 01-224 Warsaw, Poland.
  • Weglowska D; Faculty of Advanced Technologies and Chemistry, Military University of Technology, Kaliskiego Str. 2, 00-908 Warsaw, Poland.
  • Sharma PS; Institute of Physical Chemistry Polish Academy of Sciences, Kasprzaka Str. 44/52, 01-224 Warsaw, Poland.
  • Noworyta KR; Institute of Physical Chemistry Polish Academy of Sciences, Kasprzaka Str. 44/52, 01-224 Warsaw, Poland.
  • D'Souza F; Department of Chemistry, University of North Texas, 1155 Union Circle No 305070, Denton, TX 76203-5017, USA.
  • Kutner W; Institute of Physical Chemistry Polish Academy of Sciences, Kasprzaka Str. 44/52, 01-224 Warsaw, Poland.
Sensors (Basel) ; 20(17)2020 Aug 20.
Article en En | MEDLINE | ID: mdl-32825278
Liquid crystal-based sensors offer the advantage of high sensitivity at a low cost. However, they often lack selectivity altogether or require costly and unstable biomaterials to impart this selectivity. To incur this selectivity, we herein integrated a molecularly imprinted polymer (MIP) film recognition unit with a liquid crystal (LC) in an optical cell transducer. We tested the resulting chemosensor for protein determination. We examined two different LCs, each with a different optical birefringence. That way, we revealed the influence of that parameter on the sensitivity of the (human serum albumin)-templated (MIP-HSA) LC chemosensor. The response of this chemosensor with the (MIP-HSA)-recognizing film was linear from 2.2 to 15.2 µM HSA, with a limit of detection of 2.2 µM. These values are sufficient to use the devised chemosensor for HSA determination in biological samples. Importantly, the imprinting factor (IF) of this chemosensor was appreciable, reaching IF = 3.7. This IF value indicated the predominant binding of the HSA through specific rather than nonspecific interactions with the MIP.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas / Cristales Líquidos / Polímeros Impresos Molecularmente Tipo de estudio: Diagnostic_studies Límite: Humans Idioma: En Revista: Sensors (Basel) Año: 2020 Tipo del documento: Article País de afiliación: Polonia Pais de publicación: Suiza
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas / Cristales Líquidos / Polímeros Impresos Molecularmente Tipo de estudio: Diagnostic_studies Límite: Humans Idioma: En Revista: Sensors (Basel) Año: 2020 Tipo del documento: Article País de afiliación: Polonia Pais de publicación: Suiza