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Decreased Riboflavin Impregnation Time Does Not Increase the Risk for Endothelial Phototoxicity During Corneal Cross-Linking.
Marcovich, Arie L; Brekelmans, Jurriaan; Brandis, Alexander; Samish, Ilan; Pinkas, Iddo; Preise, Dina; Sasson, Keren; Feine, Ilan; Goz, Alexandra; Dickman, Mor M; Nuijts, Rudy M M A; Scherz, Avigdor.
Afiliación
  • Marcovich AL; Plant and Environmental Sciences Department, Weizmann Institute of Science, Rehovot, Israel.
  • Brekelmans J; Department of Ophthalmology, Kaplan Medical Center, Rehovot, Israel.
  • Brandis A; Plant and Environmental Sciences Department, Weizmann Institute of Science, Rehovot, Israel.
  • Samish I; Department of Ophthalmology, Maastricht University Medical Center, Maastricht, The Netherlands.
  • Pinkas I; Plant and Environmental Sciences Department, Weizmann Institute of Science, Rehovot, Israel.
  • Preise D; Biological Services Department, Weizmann Institute of Science, Rehovot, Israel.
  • Sasson K; Plant and Environmental Sciences Department, Weizmann Institute of Science, Rehovot, Israel.
  • Feine I; Plant and Environmental Sciences Department, Weizmann Institute of Science, Rehovot, Israel.
  • Goz A; Chemical Research Support, Weizmann Institute of Science, Rehovot, Israel.
  • Dickman MM; Plant and Environmental Sciences Department, Weizmann Institute of Science, Rehovot, Israel.
  • Nuijts RMMA; Plant and Environmental Sciences Department, Weizmann Institute of Science, Rehovot, Israel.
  • Scherz A; Plant and Environmental Sciences Department, Weizmann Institute of Science, Rehovot, Israel.
Transl Vis Sci Technol ; 9(6): 4, 2020 05.
Article en En | MEDLINE | ID: mdl-32821501
Purpose: To evaluate the riboflavin (RF) concentration and distribution in the corneal stroma and the risk for endothelial photodamage during corneal crosslinking (CXL) following 10- and 30-minute impregnation. Methods: De-epithelialized rabbit corneas were subjected to impregnation for 10 and 30 minutes with different RF formulations. Human corneal endothelial cells (HCECs) were subjected to different RF concentrations and ultraviolet A (UVA) dosages. Assays included fluorescence imaging, absorption spectroscopy of corneal buttons and anterior chamber humor, and cell viability staining. Results: After 10 and 30 minutes of impregnation, respectively, anterior chamber fluid showed an RF concentration of (1.6 ± 0.21)•10-4% and (5.4 ± 0.21)•10-4%, and trans-corneal absorption reported an average corneal RF concentration of 0.0266% and 0.0345%. This results in a decrease in endothelial RF concentration from 0.019% to 0.0056%, whereas endothelial UVA irradiance increases by 1.3-fold when changing from 30 to 10 minutes of impregnation. HCEC viability in cultures exposed to UVA illumination and RF concentrations as concluded for the endothelium after 10- and 30-minute impregnation was nonstatistically different at 51.0% ± 3.9 and 41.3 ± 5.0%, respectively. Conclusions: The risk for endothelial damage in CXL by RF/UVA treatment does not increase by shortened impregnation because the 30% increase in light intensity is accompanied by a 3.4-fold decrease of the RF concentration in the posterior stroma. This is substantiated by similar endothelial cell toxicity seen in vitro, which in fact appears to favor 10-minute impregnation. Translational Relevance: This study offers compelling arguments for (safely) shortening RF impregnation duration, reducing patients' burden and costly operation room time.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fármacos Fotosensibilizantes / Células Endoteliales Tipo de estudio: Etiology_studies / Risk_factors_studies Límite: Animals / Humans Idioma: En Revista: Transl Vis Sci Technol Año: 2020 Tipo del documento: Article País de afiliación: Israel Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fármacos Fotosensibilizantes / Células Endoteliales Tipo de estudio: Etiology_studies / Risk_factors_studies Límite: Animals / Humans Idioma: En Revista: Transl Vis Sci Technol Año: 2020 Tipo del documento: Article País de afiliación: Israel Pais de publicación: Estados Unidos