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Identification of a Bacillus amyloliquefaciens H6 Thioesterase Involved in Zearalenone Detoxification by Transcriptomic Analysis.
Xu, Laipeng; Sun, Xiangli; Wan, Xianhua; Li, Hui; Yan, Fengbin; Han, Ruili; Li, Hong; Li, Zhuanjian; Tian, Yadong; Liu, Xiaojun; Kang, Xiangtao; Wang, Yanbin.
Afiliación
  • Xu L; College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450000, China.
  • Sun X; College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450000, China.
  • Wan X; College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450000, China.
  • Li H; College of Environmental and Resource Sciences, Henan Agricultural University, Zhengzhou 450000, China.
  • Yan F; College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450000, China.
  • Han R; Henan Research Center of Germplasm Resources for Poultry, Zhengzhou 450002, China.
  • Li H; College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450000, China.
  • Li Z; Henan Research Center of Germplasm Resources for Poultry, Zhengzhou 450002, China.
  • Tian Y; College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450000, China.
  • Liu X; Henan Research Center of Germplasm Resources for Poultry, Zhengzhou 450002, China.
  • Kang X; College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450000, China.
  • Wang Y; Henan Research Center of Germplasm Resources for Poultry, Zhengzhou 450002, China.
J Agric Food Chem ; 68(37): 10071-10080, 2020 Sep 16.
Article en En | MEDLINE | ID: mdl-32815728
Zearalenone (ZEA), a nonsteroidal estrogenic mycotoxin produced by Fusarium graminearum, induces hyperestrogenic responses in mammals and can cause reproductive disorders in farm animals. In this study, a transcriptome analysis of Bacillus amyloliquefaciens H6, which was previously identified as a ZEA-degrading bacterium, was conducted with high-throughput sequencing technology, and the differentially expressed genes were subjected to gene ontology (GO) and kyoto encyclopedia of genes and genomes (KEGG) enrichment analyses. Among the 16 upregulated genes, BAMF_RS30125 was predicted to be the key gene responsible for ZEA degradation. The protein encoded by BAMF_RS30125 was then expressed in Escherichia coli, and this recombinant protein (named ZTE138) significantly reduced the ZEA content, as determined by the enzyme-linked immunosorbent assay (ELISA) and high-performance liquid chromatography (HPLC), and decreased the proliferating activity of ZEA in MCF-7 cells. What is more, the liquid chromatography-tandem mass spectrometry (LC-MS/MS) results showed that the relative molecular mass and the structure of ZEA also changed. Sequence alignment of the ZTE138 protein showed that it is a protease that belongs to the YBGC/FADM family of coenzyme A thioesterases, and thus, the protein can presumably cleave the ZEA lactone bond and break down its macrolide ring.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Tioléster Hidrolasas / Proteínas Bacterianas / Zearalenona / Bacillus amyloliquefaciens Tipo de estudio: Diagnostic_studies Idioma: En Revista: J Agric Food Chem Año: 2020 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Tioléster Hidrolasas / Proteínas Bacterianas / Zearalenona / Bacillus amyloliquefaciens Tipo de estudio: Diagnostic_studies Idioma: En Revista: J Agric Food Chem Año: 2020 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos