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Molecular characterization and immunohistochemical localization of tilapia piscidin 3 in response to Aeromonas hydrophila infection in Nile tilapia.
Mahrous, Karima F; Mabrouk, Dalia M; Aboelenin, Mohamad M; Abd El-Kader, Heba A M; Gaafar, Alkhateib Y; Younes, Abdelgayed M; Mahmoud, Mahmoud A; Khalil, Wagdy K B; Hassanane, Mohamed S.
Afiliación
  • Mahrous KF; Cell Biology Department, Genetic Engineering and Biotechnology Division Research, National Research Centre, 33 El Buhouth St., Dokki, Giza, 12622, Egypt.
  • Mabrouk DM; Cell Biology Department, Genetic Engineering and Biotechnology Division Research, National Research Centre, 33 El Buhouth St., Dokki, Giza, 12622, Egypt.
  • Aboelenin MM; Cell Biology Department, Genetic Engineering and Biotechnology Division Research, National Research Centre, 33 El Buhouth St., Dokki, Giza, 12622, Egypt.
  • Abd El-Kader HAM; Cell Biology Department, Genetic Engineering and Biotechnology Division Research, National Research Centre, 33 El Buhouth St., Dokki, Giza, 12622, Egypt.
  • Gaafar AY; Hydrobiology Department, Veterinary Research Division, National Research Centre, 33 El Buhouth St., Dokki, Giza, 12622, Egypt.
  • Younes AM; Hydrobiology Department, Veterinary Research Division, National Research Centre, 33 El Buhouth St., Dokki, Giza, 12622, Egypt.
  • Mahmoud MA; Pathology Department, Faculty of Veterinary Medicine, Cairo University, Giza, 12211, Egypt.
  • Khalil WKB; Cell Biology Department, Genetic Engineering and Biotechnology Division Research, National Research Centre, 33 El Buhouth St., Dokki, Giza, 12622, Egypt.
  • Hassanane MS; Cell Biology Department, Genetic Engineering and Biotechnology Division Research, National Research Centre, 33 El Buhouth St., Dokki, Giza, 12622, Egypt.
J Pept Sci ; 26(11): e3280, 2020 Nov.
Article en En | MEDLINE | ID: mdl-32812302
The antimicrobial activity of tilapia piscidin 3 (TP3) was determined in vitro against a locally isolated Aeromonas hydrophila. A 388 bp fragment was amplified from the TP3 cDNA and sequenced. The coding sequence (CDS) of TP3 was estimated to be 231 bp codes for 76 amino acids long and stop codon. In silico analysis was performed to detect both the signal peptide and the prodomain cleavage sites to follow the amino acids number 22 and 70, respectively. Based on this, a peptide 23 amino acids long with a remarkably high computed antimicrobial probability was synthesized and used in the subsequent experiments. The antimicrobial activity of TP3 was determined with minimum inhibitory concentration (MIC) and minim um bactericidal concentration (MBC) methods. TP3 exhibited relatively weak antimicrobial activities against the tested bacteria. A challenge experiment was then performed in Nile tilapia with low and high doses of A. hydrophila, followed by timely recognition; after 3, 6, 24 h, and 7 days of the specific TP3 gene expression, immunohistochemical localization was also performed. Histopathological examination revealed provoked inflammatory responses and congestion in the same organs of TP3 expression. Immunohistochemical localization showed that A. hydrophila induced tilapia fish to express TP3 after 24 h within the gills, intestine, hepatopancreas, spleen, and posterior kidney. In quantitative real time (RT)-polymerase chain reaction analysis, the high dose showed higher mRNA expression levels than the low dose, and its expression levels increased in the A. hydrophila-infected fish. It was therefore concluded that TP3 plays an essential role in fish immunity.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Infecciones por Bacterias Gramnegativas / Aeromonas hydrophila / Péptidos Catiónicos Antimicrobianos / Enfermedades de los Peces / Antibacterianos Límite: Animals Idioma: En Revista: J Pept Sci Asunto de la revista: BIOQUIMICA Año: 2020 Tipo del documento: Article País de afiliación: Egipto Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Infecciones por Bacterias Gramnegativas / Aeromonas hydrophila / Péptidos Catiónicos Antimicrobianos / Enfermedades de los Peces / Antibacterianos Límite: Animals Idioma: En Revista: J Pept Sci Asunto de la revista: BIOQUIMICA Año: 2020 Tipo del documento: Article País de afiliación: Egipto Pais de publicación: Reino Unido