Mammalian orthoreovirus core protein µ2 reorganizes host microtubule-organizing center components.
Virology
; 549: 13-24, 2020 10.
Article
en En
| MEDLINE
| ID: mdl-32805585
Filamentous mammalian orthoreovirus (MRV) viral factories (VFs) are membrane-less cytosolic inclusions in which virus transcription, replication of dsRNA genome segments, and packaging of virus progeny into newly synthesized virus cores take place. In infected cells, the MRV µ2 protein forms punctae in the enlarged region of the filamentous VFs that are co-localized with γ-tubulin and resistant to nocodazole treatment, and permitted microtubule (MT)-extension, features common to MT-organizing centers (MTOCs). Using a previously established reconstituted VF model, we addressed the functions of MT-components and MTOCs concerning their roles in the formation of filamentous VFs. Indeed, the MTOC markers γ-tubulin and centrin were redistributed within the VF-like structures (VFLS) in a µ2-dependent manner. Moreover, the MT-nucleation centers significantly increased in numbers, and γ-tubulin was pulled-down in a binding assay when co-expressed with histidine-tagged-µ2 and µNS. Thus, µ2, by interaction with γ-tubulin, can modulate MTOCs localization and function according to viral needs.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Tubulina (Proteína)
/
Proteínas Virales
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Centro Organizador de los Microtúbulos
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Orthoreovirus de los Mamíferos
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Interacciones Huésped-Patógeno
/
Microtúbulos
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
Virology
Año:
2020
Tipo del documento:
Article
Pais de publicación:
Estados Unidos