Enhanced Heterologous Production of Glycosyltransferase UGT76G1 by Co-Expression of Endogenous prpD and malK in Escherichia coli and Its Transglycosylation Application in Production of Rebaudioside.

Shu, Wenju; Zheng, Hongchen; Fu, Xiaoping; Zhen, Jie; Tan, Ming; Xu, Jianyong; Zhao, Xingya; Yang, Shibin; Song, Hui; Ma, Yanhe

Enhanced Heterologous Production of Glycosyltransferase UGT76G1 by Co-Expression of Endogenous prpD and malK in Escherichia coli and Its Transglycosylation Application in Production of Rebaudioside.

Shu, Wenju; Zheng, Hongchen; Fu, Xiaoping; Zhen, Jie; Tan, Ming; Xu, Jianyong; Zhao, Xingya; Yang, Shibin; Song, Hui; Ma, Yanhe.

Afiliación

Shu W; University of Chinese Academy of Sciences, Beijing 100049, China.
Zheng H; Industrial Enzymes National Engineering Laboratory, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China.
Fu X; University of Chinese Academy of Sciences, Beijing 100049, China.
Zhen J; Industrial Enzymes National Engineering Laboratory, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China.
Tan M; Tianjin Key Laboratory for Industrial Biological Systems and Bioprocessing Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China.
Xu J; Industrial Enzymes National Engineering Laboratory, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China.
Zhao X; Tianjin Key Laboratory for Industrial Biological Systems and Bioprocessing Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China.
Yang S; Industrial Enzymes National Engineering Laboratory, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China.
Song H; Tianjin Key Laboratory for Industrial Biological Systems and Bioprocessing Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China.
Ma Y; Industrial Enzymes National Engineering Laboratory, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China.

Int J Mol Sci ; 21(16)2020 Aug 11.

Article en En | MEDLINE | ID: mdl-32796599

Asunto(s)

Transportadoras de Casetes de Unión a ATP/metabolismo; Proteínas de Escherichia coli/metabolismo; Escherichia coli/metabolismo; Glicósidos/biosíntesis; Glicosiltransferasas/metabolismo; Hidroliasas/metabolismo; Biocatálisis; Reactores Biológicos/microbiología; Biotransformación; Fermentación; Glicósidos/química; Glicosilación; Plásmidos/metabolismo; Proteínas Recombinantes de Fusión/metabolismo; Recombinación Genética/genética; Solubilidad

Palabras clave

co-expression; efficient E. coli expression system; enzymatic biotransformation; fusion partner; malK; prpD; steviol glycosides; uridine diphosphate dependent glucosyltransferases (UGTs)

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Glicosiltransferasas / Transportadoras de Casetes de Unión a ATP / Proteínas de Escherichia coli / Escherichia coli / Glicósidos / Hidroliasas Idioma: En Revista: Int J Mol Sci Año: 2020 Tipo del documento: Article País de afiliación: China Pais de publicación: Suiza

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