Efficient long fragment editing technique enables large-scale and scarless bacterial genome engineering.

Huang, Chaoyong; Guo, Liwei; Wang, Jingge; Wang, Ning; Huo, Yi-Xin

Huang, Chaoyong; Guo, Liwei; Wang, Jingge; Wang, Ning; Huo, Yi-Xin.

Afiliación

Huang C; Key Laboratory of Molecular Medicine and Biotherapy, School of Life Science, Beijing Institute of Technology, Beijing, 100081, China.
Guo L; SIP-UCLA Institute for Technology Advancement, Suzhou, 215123, China.
Wang J; Key Laboratory of Molecular Medicine and Biotherapy, School of Life Science, Beijing Institute of Technology, Beijing, 100081, China.
Wang N; Key Laboratory of Molecular Medicine and Biotherapy, School of Life Science, Beijing Institute of Technology, Beijing, 100081, China.
Huo YX; Key Laboratory of Molecular Medicine and Biotherapy, School of Life Science, Beijing Institute of Technology, Beijing, 100081, China.

Appl Microbiol Biotechnol ; 104(18): 7943-7956, 2020 Sep.

Article en En | MEDLINE | ID: mdl-32794018

Asunto(s)

Sistemas CRISPR-Cas; Escherichia coli; Escherichia coli/genética; Edición Génica; Ingeniería Genética; Genoma Bacteriano; Ingeniería Metabólica

Palabras clave

Genetic stability; Genome engineering; Genome simplification; Long fragment editing; Metabolic engineering

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Escherichia coli / Sistemas CRISPR-Cas Idioma: En Revista: Appl Microbiol Biotechnol Año: 2020 Tipo del documento: Article País de afiliación: China Pais de publicación: Alemania

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