Crystallographic and cryogenic electron microscopic structures and enzymatic characterization of sulfur oxygenase reductase from <i>Sulfurisphaera tokodaii</i>.

Sato, Yuta; Yabuki, Takashi; Adachi, Naruhiko; Moriya, Toshio; Arakawa, Takatoshi; Kawasaki, Masato; Yamada, Chihaya; Senda, Toshiya; Fushinobu, Shinya; Wakagi, Takayoshi

Crystallographic and cryogenic electron microscopic structures and enzymatic characterization of sulfur oxygenase reductase from Sulfurisphaera tokodaii.

Sato, Yuta; Yabuki, Takashi; Adachi, Naruhiko; Moriya, Toshio; Arakawa, Takatoshi; Kawasaki, Masato; Yamada, Chihaya; Senda, Toshiya; Fushinobu, Shinya; Wakagi, Takayoshi.

Afiliación

Sato Y; Department of Biotechnology, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
Yabuki T; Department of Biotechnology, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
Adachi N; Structural Biology Research Center, Institute of Materials Structure Science, High Energy Accelerator Research Organization (KEK), 1-1 Oho, Tsukuba, Ibaraki 305-0801, Japan.
Moriya T; Structural Biology Research Center, Institute of Materials Structure Science, High Energy Accelerator Research Organization (KEK), 1-1 Oho, Tsukuba, Ibaraki 305-0801, Japan.
Arakawa T; Department of Biotechnology, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
Kawasaki M; Collaborative Research Institute for Innovative Microbiology, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
Yamada C; Structural Biology Research Center, Institute of Materials Structure Science, High Energy Accelerator Research Organization (KEK), 1-1 Oho, Tsukuba, Ibaraki 305-0801, Japan.
Senda T; Department of Biotechnology, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
Fushinobu S; Collaborative Research Institute for Innovative Microbiology, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
Wakagi T; Structural Biology Research Center, Institute of Materials Structure Science, High Energy Accelerator Research Organization (KEK), 1-1 Oho, Tsukuba, Ibaraki 305-0801, Japan.

J Struct Biol X ; 4: 100030, 2020.

Article en En | MEDLINE | ID: mdl-32775998

Palabras clave

AaSOR, Acidianus ambivalens SOR; AqSOR, Aquifex aeolicus SOR; Archaea; AtSOR, Acidianus tengchongensis SOR; CTF, contrast transfer function; Cryogenic electron microscopy; DTNB, 5,5'-dithiobis(2-nitrobenzoic acid); FSC, Fourier shell correlation; HnSOR, Halothiobacillus neapolitanus SOR; Nonheme mononuclear iron center; PAGE, polyacrylamide gel electrophoresis; RMSD, root mean square deviation; SD, standard deviation; SDS, sodium dodecyl sulfate; SOR, sulfur oxygenase reductase; SbSOR, Sulfobacillus thermosulfidooxidans SOR; StSOR, Sulfurisphaera tokodaii SOR; Sulfur metabolism; TpSOR, Thioalkalivibrio paradoxus SOR; X-ray crystallography; cryo-EM, cryogenic electron microscopy; pCMB, p-chloromercuribenzoate

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: J Struct Biol X Año: 2020 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Estados Unidos

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