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Discovery of Driver Genes in Colorectal HT29-derived Cancer Stem-Like Tumorspheres.
Cheng, Chun-Chia; Hsu, Po-Jui; Sie, Zong-Lin; Chen, Fang-Hsin.
Afiliación
  • Cheng CC; Radiation Biology Research Center, Institute for Radiological Research, Chang Gung University, Chang Gung Memorial Hospital at Linkou.
  • Hsu PJ; Department of Laboratory Medicine, Mackay Memorial Hospital.
  • Sie ZL; Radiation Biology Research Center, Institute for Radiological Research, Chang Gung University, Chang Gung Memorial Hospital at Linkou.
  • Chen FH; Radiation Biology Research Center, Institute for Radiological Research, Chang Gung University, Chang Gung Memorial Hospital at Linkou; Department of Medical Imaging and Radiological Sciences, Chang Gung University; Department of Radiation Oncology, Chang Gung Memorial Hospital; fanghsin0808@gmail.co
J Vis Exp ; (161)2020 07 22.
Article en En | MEDLINE | ID: mdl-32773762
Cancer stem cells play a vital role against clinical therapies, contributing to tumor relapse. There are many oncogenes involved in tumorigenesis and the initiation of cancer stemness properties. Since gene expression in the formation of colorectal cancer-derived tumorspheres is unclear, it takes time to discover the mechanisms working on one gene at a time. This study demonstrates a method to quickly discover the driver genes involved in the survival of the colorectal cancer stem-like cells in vitro. Colorectal HT29 cancer cells that express the LGR5 when cultured as spheroids and accompany an increase CD133 stemness markers were selected and used in this study. The protocol presented is used to perform RNAseq with available bioinformatics to quickly uncover the overexpressed driver genes in the formation of colorectal HT29-derived stem-like tumorspheres. The methodology can quickly screen and discover potential driver genes in other disease models.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Células Madre Neoplásicas / Neoplasias Colorrectales / Esferoides Celulares Tipo de estudio: Guideline Límite: Humans Idioma: En Revista: J Vis Exp Año: 2020 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Células Madre Neoplásicas / Neoplasias Colorrectales / Esferoides Celulares Tipo de estudio: Guideline Límite: Humans Idioma: En Revista: J Vis Exp Año: 2020 Tipo del documento: Article Pais de publicación: Estados Unidos