Your browser doesn't support javascript.
loading
Delivery of hepatocyte growth factor mRNA from nanofibrillar scaffolds in a pig model of peripheral arterial disease.
Zaitseva, Tatiana S; Yang, Guang; Dionyssiou, Dimitris; Zamani, Maedeh; Sawamura, Steve; Yakubov, Eduard; Ferguson, James; Hallett, Richard L; Fleischmann, Dominik; Paukshto, Michael V; Huang, Ngan F.
Afiliación
  • Zaitseva TS; Fibralign Corporation, Union City, CA 94587, USA.
  • Yang G; Stanford Cardiovascular Institute, Stanford, CA 94305, USA.
  • Dionyssiou D; Veterans Affairs Palo Alto Health Care System, Palo Alto, CA 94304,USA.
  • Zamani M; Fibralign Corporation, Union City, CA 94587, USA.
  • Sawamura S; Department of Plastic Surgery, Aristotle University of Thessaloniki, Thessaloniki 54124, Greece.
  • Yakubov E; Stanford Cardiovascular Institute, Stanford, CA 94305, USA.
  • Ferguson J; Department of Cardiothoracic Surgery, Stanford University, Stanford, CA 94305, USA.
  • Hallett RL; Fibralign Corporation, Union City, CA 94587, USA.
  • Fleischmann D; PhaRNA, LLC, Houston, TX 77024, USA.
  • Paukshto MV; Palo Alto Bioresources, Berkeley, CA 94705, USA.
  • Huang NF; Stanford Cardiovascular Institute, Stanford, CA 94305, USA.
Regen Med ; 15(6): 1761-1773, 2020 06.
Article en En | MEDLINE | ID: mdl-32772903
Background: Chemical modification of mRNA (mmRNA) substantially improves their stability and translational efficiency within cells. Nanofibrillar collagen scaffolds were previously shown to enable the spatially localized delivery and temporally controlled release of mmRNA encoding HGF both in vitro and in vivo. Materials & methods: Herein we developed an improved slow-releasing HGF mmRNA scaffold and tested its therapeutic efficacy in a porcine model of peripheral arterial disease. Results & conclusion: The HGF mmRNA was released from scaffolds in a temporally controlled fashion in vitro with preserved transfection activity. The mmRNA scaffolds improved vascular regeneration when sutured to the ligated porcine femoral artery. These studies validate the therapeutic potential of HGF mmRNA delivery from nanofibrillar scaffolds for treatment of peripheral arterial disease.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: ARN Mensajero / Factor de Crecimiento de Hepatocito / Modelos Animales de Enfermedad / Andamios del Tejido / Enfermedad Arterial Periférica / Miembro Posterior / Isquemia Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Regen Med Año: 2020 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: ARN Mensajero / Factor de Crecimiento de Hepatocito / Modelos Animales de Enfermedad / Andamios del Tejido / Enfermedad Arterial Periférica / Miembro Posterior / Isquemia Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Regen Med Año: 2020 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido