Your browser doesn't support javascript.
loading
An in situ collagen-HA hydrogel system promotes survival and preserves the proangiogenic secretion of hiPSC-derived vascular smooth muscle cells.
Dash, Biraja C; Duan, Kaiti; Xing, Hao; Kyriakides, Themis R; Hsia, Henry C.
Afiliación
  • Dash BC; Section of Plastic Surgery, Department of Surgery, Yale School of Medicine, Yale University, New Haven, Connecticut.
  • Duan K; Section of Plastic Surgery, Department of Surgery, Yale School of Medicine, Yale University, New Haven, Connecticut.
  • Xing H; Department of Biomedical Engineering, Yale University, New Haven, Connecticut.
  • Kyriakides TR; Department of Biomedical Engineering, Yale University, New Haven, Connecticut.
  • Hsia HC; Department of Pathology, Yale University, New Haven, Connecticut.
Biotechnol Bioeng ; 117(12): 3912-3923, 2020 12.
Article en En | MEDLINE | ID: mdl-32770746
Human-induced pluripotent stem cell-derived vascular smooth muscle cells (hiPSC-VSMCs) with proangiogenic properties have huge therapeutic potential. While hiPSC-VSMCs have already been utilized for wound healing using a biomimetic collagen scaffold, an in situ forming hydrogel mimicking the native environment of skin offers the promise of hiPSC-VSMC mediated repair and regeneration. Herein, the impact of a collagen type-I-hyaluronic acid (HA) in situ hydrogel cross-linked using a polyethylene glycol-based cross-linker on hiPSC-VSMCs viability and proangiogenic paracrine secretion was investigated. Our study demonstrated increases in cell viability, maintenance of phenotype and proangiogenic growth factor secretion, and proangiogenic activity in response to the conditioned medium. The optimally cross-linked and functionalized collagen type-I/HA hydrogel system developed in this study shows promise as an in situ hiPSC-VSMC carrier system for wound regeneration.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Colágeno / Hidrogeles / Miocitos del Músculo Liso / Células Madre Pluripotentes Inducidas / Ácido Hialurónico / Músculo Liso Vascular Límite: Humans Idioma: En Revista: Biotechnol Bioeng Año: 2020 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Colágeno / Hidrogeles / Miocitos del Músculo Liso / Células Madre Pluripotentes Inducidas / Ácido Hialurónico / Músculo Liso Vascular Límite: Humans Idioma: En Revista: Biotechnol Bioeng Año: 2020 Tipo del documento: Article Pais de publicación: Estados Unidos