Gene structure, evolution and expression analysis of the P-ATPase gene family in Chinese pear (Pyrus bretschneideri).

Manzoor, Muhammad Aamir; Cheng, Xi; Li, Guohui; Su, Xueqiang; Abdullah, Muhammad; Cai, Yongping

Manzoor, Muhammad Aamir; Cheng, Xi; Li, Guohui; Su, Xueqiang; Abdullah, Muhammad; Cai, Yongping.

Afiliación

Manzoor MA; School of Life Sciences, Anhui Agricultural University, Hefei 230036, China.
Cheng X; School of Life Sciences, Anhui Agricultural University, Hefei 230036, China.
Li G; School of Life Sciences, Anhui Agricultural University, Hefei 230036, China.
Su X; School of Life Sciences, Anhui Agricultural University, Hefei 230036, China.
Abdullah M; School of Agriculture and Biology, Shanghai Jiao tong University Agricultural University, Shanghai, China; School of Life Sciences, Anhui Agricultural University, Hefei 230036, China.
Cai Y; School of Life Sciences, Anhui Agricultural University, Hefei 230036, China. Electronic address: swkx12@ahau.edu.cn.

Comput Biol Chem ; 88: 107346, 2020 Oct.

Article en En | MEDLINE | ID: mdl-32759051

RESUMEN

P-ATPase are a large protein family of integral membrane, playing an important role in plant growth, development and stress. P-ATPase genes family have been identified and characterized in several model plants such as cotton, grapes, tobacco, rice, rubber plant and Arabidopsis. However, still lack of comprehensive study of P-ATPase genes in Chinese pear (Pyrus bretschneideri). A systematic analysis was performed and identified 30 P-ATPase genes from the pear genome to evaluate the qualities and diversity of P-ATPase proteins. Phylogenetic analysis was performed using A. thaliana P-ATPase genes as a model, allowing us to categorize into 4 subfamilies (PbHMA, PbECA, PbACA, and PbAHA) and two subfamilies (ALA and P5) is absent in pear. Even Within the same subclade, P-ATPase genes also shows the similar exon-intron structure and conserved motif structure. Continuing chromosomal localization analysis showed that 23 P-ATPase genes were distributed among 13 chromosome and 7 gene on the scaffold of pear. Promoter regions of P-ATPase genes revealed that several cis-acting elements were involved in plant growth/development, stress responses as well as hormone responses. Additionally, P-ATPase genes were also differentially expressed under hormones treatments of ABA (abscisic acid) and SA (salicylic acid) treatments. Remarkably, the transcriptome data exposed that P-ATPase gene might play an important role in lignin biosynthesis during fruit development. The real time qRT-PCR was performed, and the expression analysis indicated that various P-ATPase genes extremely expressed during different developmental stages of fruit. Our study provides valuable information about the P-ATPase gene family in pear fruit development and lignin polymerization.

Asunto(s)

Adenosina Trifosfatasas/genética; Genes de Plantas/genética; Proteínas de Plantas/genética; Pyrus/enzimología; Adenosina Trifosfatasas/metabolismo; Proteínas de Plantas/metabolismo

Palabras clave

Evolution; Expression pattern; P-ATPase; Phylogenetic analysis; qRT-PCR

Texto completo

Añadir a Mi BVS

Imprimir

XML

PubMed Links

Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas de Plantas / Genes de Plantas / Adenosina Trifosfatasas / Pyrus Idioma: En Revista: Comput Biol Chem Asunto de la revista: BIOLOGIA / INFORMATICA MEDICA / QUIMICA Año: 2020 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido

Texto completo

Añadir a Mi BVS

Imprimir

XML

PubMed Links

Buscar en Google