Your browser doesn't support javascript.
loading
ARID1a Associates with Lymphoid-Restricted Transcription Factors and Has an Essential Role in T Cell Development.
Astori, Audrey; Tingvall-Gustafsson, Johanna; Kuruvilla, Jacob; Coyaud, Etienne; Laurent, Estelle M N; Sunnerhagen, Maria; Åhsberg, Josefine; Ungerbäck, Jonas; Strid, Tobias; Sigvardsson, Mikael; Raught, Brian; Somasundaram, Rajesh.
Afiliación
  • Astori A; Princess Margaret Cancer Centre, University Health Network, Toronto, Ontario M5G 1L7, Canada.
  • Tingvall-Gustafsson J; Division of Molecular Hematology, Lund University, 22184 Lund, Sweden.
  • Kuruvilla J; Department of Biomedical and Clinical Sciences, Linköping University, 581 85 Linköping, Sweden.
  • Coyaud E; Princess Margaret Cancer Centre, University Health Network, Toronto, Ontario M5G 1L7, Canada.
  • Laurent EMN; Princess Margaret Cancer Centre, University Health Network, Toronto, Ontario M5G 1L7, Canada.
  • Sunnerhagen M; Department of Physics, Chemistry and Biology, Linköping University, 581 83 Linköping, Sweden; and.
  • Åhsberg J; Department of Biomedical and Clinical Sciences, Linköping University, 581 85 Linköping, Sweden.
  • Ungerbäck J; Division of Molecular Hematology, Lund University, 22184 Lund, Sweden.
  • Strid T; Department of Biomedical and Clinical Sciences, Linköping University, 581 85 Linköping, Sweden.
  • Sigvardsson M; Division of Molecular Hematology, Lund University, 22184 Lund, Sweden; mikael.sigvardsson@med.lu.se.
  • Raught B; Department of Biomedical and Clinical Sciences, Linköping University, 581 85 Linköping, Sweden.
  • Somasundaram R; Princess Margaret Cancer Centre, University Health Network, Toronto, Ontario M5G 1L7, Canada.
J Immunol ; 205(5): 1419-1432, 2020 09 01.
Article en En | MEDLINE | ID: mdl-32747500
Maturation of lymphoid cells is controlled by the action of stage and lineage-restricted transcription factors working in concert with the general transcription and chromatin remodeling machinery to regulate gene expression. To better understand this functional interplay, we used Biotin Identification in human embryonic kidney cells to identify proximity interaction partners for GATA3, TCF7 (TCF1), SPI1, HLF, IKZF1, PAX5, ID1, and ID2. The proximity interaction partners shared among the lineage-restricted transcription factors included ARID1a, a BRG1-associated factor complex component. CUT&RUN analysis revealed that ARID1a shared binding with TCF7 and GATA3 at a substantial number of putative regulatory elements in mouse T cell progenitors. In support of an important function for ARID1a in lymphocyte development, deletion of Arid1a in early lymphoid progenitors in mice resulted in a pronounced developmental arrest in early T cell development with a reduction of CD4+CD8+ cells and a 20-fold reduction in thymic cellularity. Exploring gene expression patterns in DN3 cells from Wt and Arid1a-deficient mice suggested that the developmental block resided in the DN3a to DN3b transition, indicating a deficiency in ß-selection. Our work highlights the critical importance of functional interactions between stage and lineage-restricted factors and the basic transcription machinery during lymphocyte differentiation.
Asunto(s)
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Factores de Transcripción / Linfocitos / Linfocitos T CD4-Positivos / Linfocitos T CD8-positivos / Proteínas de Unión al ADN Tipo de estudio: Prognostic_studies / Risk_factors_studies Límite: Animals / Humans Idioma: En Revista: J Immunol Año: 2020 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Factores de Transcripción / Linfocitos / Linfocitos T CD4-Positivos / Linfocitos T CD8-positivos / Proteínas de Unión al ADN Tipo de estudio: Prognostic_studies / Risk_factors_studies Límite: Animals / Humans Idioma: En Revista: J Immunol Año: 2020 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Estados Unidos