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Role of HPV 16 variants among cervical carcinoma samples from Northeastern Brazil.
da Silva, Rodrigo Lopes; da Silva Batista, Zulmira; Bastos, Gerusinete Rodrigues; Cunha, Ana Paula Almeida; Figueiredo, Fábio Vidal; de Castro, Lailson Oliveira; Dos Anjos Pereira, Liwerbeth; da Silva, Marcos Antonio Custódio Neto; Vidal, Flávia Castello Branco; Barros, Maria Claudene; da Costa Fraga, Elmary; Brito, Luciane Maria Oliveira; do Carmo Lacerda Barbosa, Maria; Moreira, Miguel Ângelo Martins; do Desterro Soares Brandão Nascimento, Maria.
Afiliación
  • da Silva RL; Federal University of Maranhão (UFMA), São Luís, Maranhão, Brazil.
  • da Silva Batista Z; Federal University of Maranhão (UFMA), São Luís, Maranhão, Brazil.
  • Bastos GR; Federal University of Maranhão (UFMA), São Luís, Maranhão, Brazil.
  • Cunha APA; Federal University of Maranhão (UFMA), São Luís, Maranhão, Brazil.
  • Figueiredo FV; Federal University of Maranhão (UFMA), São Luís, Maranhão, Brazil.
  • de Castro LO; Faculty of Medicine, Federal University of Maranhão (UFMA), São Luís, Maranhão, Brazil.
  • Dos Anjos Pereira L; Faculty of Medicine, Federal University of Maranhão (UFMA), São Luís, Maranhão, Brazil.
  • da Silva MACN; Faculty of Medical Sciences (FCM), State University of Campinas (UNICAMP), Campinas, São Paulo, Brazil.
  • Vidal FCB; Federal University of Maranhão (UFMA), São Luís, Maranhão, Brazil.
  • Barros MC; Center of Advanced Studies of Caxias (CESC), State University of Maranhão (UEMA), Caxias, Maranhão, Brazil.
  • da Costa Fraga E; Center of Advanced Studies of Caxias (CESC), State University of Maranhão (UEMA), Caxias, Maranhão, Brazil.
  • Brito LMO; Federal University of Maranhão (UFMA), São Luís, Maranhão, Brazil.
  • do Carmo Lacerda Barbosa M; Federal University of Maranhão (UFMA), São Luís, Maranhão, Brazil.
  • Moreira MÂM; Genetic Division, National Institute of Cancer (INCA), Rio de Janeiro, Brazil.
  • do Desterro Soares Brandão Nascimento M; Federal University of Maranhão (UFMA), São Luís, Maranhão, Brazil. cnsd_ma@uol.com.br.
BMC Womens Health ; 20(1): 162, 2020 08 01.
Article en En | MEDLINE | ID: mdl-32738888
BACKGROUND: Cervical cancer is the fourth most common type of cancer affecting women globally. In Brazil, it is the third most frequent type of cancer in women and HPV is present in approximately 90% of cases. Evidence suggests that variants of HPV 16 can interfere biologically and etiologically during the development of cervical cancer. METHODS: Cervix tumor fragments were collected, their DNA was extracted, and nested PCR was used to detect HPV. Positive samples were sequenced to determine the viral genotype. To characterize the HPV 16 strains, positive samples PCR was used to amplify the LCR and E6 regions of the HPV 16 virus. RESULTS: Data from 120 patients with cervical cancer were analyzed. Most women were between 41 and 54 years of age, had schooling until primary school, a family income between 1 and 2 times the minimum wage and were married/in a consensual union. There was no statistically significant association between HPV or socio-demographic variables and risk factors for cervical cancer (P <  0.05). HPV was present in 88 women (73%). The most prevalent types were HPV 16 (53.4%), HPV 18 (13.8%), HPV 35 (6.9%) and HPV 45 (5.7%). Of the 47 HPV 16 positive cases, variant A (49%) was present in 23 samples, followed by variant D in 20 cases (43%), and variants B and C in 2 cases each (4%). The most prevalent histological type of HPV 16 tumors was squamous cell carcinoma, followed by adenocarcinoma. There was a statistically significant association between HPV 16 variants and the tumors' histological types (P <  0.001). CONCLUSIONS: Knowledge of HPV 16 variants will provide data on their influence on the pathological and oncogenic aspects of cervical lesions.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Papillomaviridae / ADN Viral / Neoplasias del Cuello Uterino / Infecciones por Papillomavirus / Papillomavirus Humano 16 Tipo de estudio: Observational_studies / Risk_factors_studies Límite: Adult / Aged / Aged80 / Female / Humans / Middle aged País/Región como asunto: America do sul / Brasil Idioma: En Revista: BMC Womens Health Asunto de la revista: SAUDE DA MULHER Año: 2020 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Reino Unido
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Papillomaviridae / ADN Viral / Neoplasias del Cuello Uterino / Infecciones por Papillomavirus / Papillomavirus Humano 16 Tipo de estudio: Observational_studies / Risk_factors_studies Límite: Adult / Aged / Aged80 / Female / Humans / Middle aged País/Región como asunto: America do sul / Brasil Idioma: En Revista: BMC Womens Health Asunto de la revista: SAUDE DA MULHER Año: 2020 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Reino Unido