Your browser doesn't support javascript.
loading
Detection of specific Atlantic salmon antibodies against salmonid alphavirus using a bead-based immunoassay.
Teige, Lena Hammerlund; Aksnes, Ida; Røsæg, Magnus Vikan; Jensen, Ingvill; Jørgensen, Jorunn; Sindre, Hilde; Collins, Catherine; Collet, Bertrand; Rimstad, Espen; Dahle, Maria K; Boysen, Preben.
Afiliación
  • Teige LH; Faculty of Veterinary Medicine, Norwegian University of Life Sciences (NMBU), Oslo, Norway.
  • Aksnes I; Faculty of Veterinary Medicine, Norwegian University of Life Sciences (NMBU), Oslo, Norway.
  • Røsæg MV; SalMar Farming AS, Trondheim, Norway.
  • Jensen I; The Norwegian College of Fishery Science, UiT The Arctic University of Norway, Tromsø, Norway.
  • Jørgensen J; The Norwegian College of Fishery Science, UiT The Arctic University of Norway, Tromsø, Norway.
  • Sindre H; Department of Fish Health, Norwegian Veterinary Institute, Oslo, Norway.
  • Collins C; Université Paris-Saclay, INRAE, UVSQ, VIM, 78350, Jouy-en-Josas, France.
  • Collet B; Université Paris-Saclay, INRAE, UVSQ, VIM, 78350, Jouy-en-Josas, France.
  • Rimstad E; Faculty of Veterinary Medicine, Norwegian University of Life Sciences (NMBU), Oslo, Norway.
  • Dahle MK; The Norwegian College of Fishery Science, UiT The Arctic University of Norway, Tromsø, Norway; Department of Fish Health, Norwegian Veterinary Institute, Oslo, Norway.
  • Boysen P; Faculty of Veterinary Medicine, Norwegian University of Life Sciences (NMBU), Oslo, Norway. Electronic address: preben.boysen@nmbu.no.
Fish Shellfish Immunol ; 106: 374-383, 2020 Nov.
Article en En | MEDLINE | ID: mdl-32738513
Salmonid alphavirus (SAV) is the etiological cause of pancreas disease (PD) in Atlantic salmon (Salmo salar). Several vaccines against SAV are in use, but PD still cause significant mortality and concern in European aquaculture, raising the need for optimal tools to monitor SAV immunity. To monitor and control the distribution of PD in Norway, all salmonid farms are regularly screened for SAV by RT-qPCR. While the direct detection of SAV is helpful in the early stages of infection, serological methods could bring additional information on acquired SAV immunity in the later stages. Traditionally, SAV antibodies are monitored in neutralization assays, but they are time-consuming and cumbersome, thus alternative assays are warranted. Enzyme-linked immunosorbent assays (ELISAs) have not yet been successfully used for anti-SAV antibody detection in aquaculture. We aimed to develop a bead-based immunoassay for SAV-specific antibodies. By using detergent-treated SAV particles as antigens, we detected SAV-specific antibodies in plasma collected from both a SAV challenge trial and a field outbreak of PD. Increased levels of SAV-specific antibodies were seen after most fish had become negative for viral RNA. The bead-based assay is time saving compared to virus neutralization assays, and suitable for non-lethal testing due to low sample size requirements. We conclude that the bead-based immunoassay for SAV antibody detection is a promising diagnostic tool to complement SAV screening in aquaculture.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enfermedades Pancreáticas / Infecciones por Alphavirus / Salmo salar / Enfermedades de los Peces Tipo de estudio: Diagnostic_studies Límite: Animals Idioma: En Revista: Fish Shellfish Immunol Asunto de la revista: BIOLOGIA / MEDICINA VETERINARIA Año: 2020 Tipo del documento: Article País de afiliación: Noruega Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enfermedades Pancreáticas / Infecciones por Alphavirus / Salmo salar / Enfermedades de los Peces Tipo de estudio: Diagnostic_studies Límite: Animals Idioma: En Revista: Fish Shellfish Immunol Asunto de la revista: BIOLOGIA / MEDICINA VETERINARIA Año: 2020 Tipo del documento: Article País de afiliación: Noruega Pais de publicación: Reino Unido