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Flavin adenine dinucleotide ameliorates hypertensive vascular remodeling via activating short chain acyl-CoA dehydrogenase.
Zhong, Xiaoyi; Ma, Zhichao; Su, Yongshao; Li, Zhonghong; Liao, Yingqin; Pan, Xuediao; Zang, Linquan; Zhou, Sigui.
Afiliación
  • Zhong X; Department of Clinical Pharmacy, GuangDong Pharmaceutical University, GuangZhou 510006, China; Guangzhou Key Laboratory of Construction and Application of New Drug Screening Model Systems, Guangdong Pharmaceutical University, Guangzhou 510006, China; Key Laboratory of New Drug Discovery and Evaluati
  • Ma Z; Department of Clinical Pharmacy, GuangDong Pharmaceutical University, GuangZhou 510006, China.
  • Su Y; Department of Clinical Pharmacy, GuangDong Pharmaceutical University, GuangZhou 510006, China.
  • Li Z; Department of Clinical Pharmacy, GuangDong Pharmaceutical University, GuangZhou 510006, China.
  • Liao Y; Department of Clinical Pharmacy, GuangDong Pharmaceutical University, GuangZhou 510006, China.
  • Pan X; Department of Clinical Pharmacy, GuangDong Pharmaceutical University, GuangZhou 510006, China.
  • Zang L; Department of Clinical Pharmacy, GuangDong Pharmaceutical University, GuangZhou 510006, China.
  • Zhou S; Department of Clinical Pharmacy, GuangDong Pharmaceutical University, GuangZhou 510006, China; Guangzhou Key Laboratory of Construction and Application of New Drug Screening Model Systems, Guangdong Pharmaceutical University, Guangzhou 510006, China; Key Laboratory of New Drug Discovery and Evaluati
Life Sci ; 258: 118156, 2020 Oct 01.
Article en En | MEDLINE | ID: mdl-32735886
AIMS: Flavin adenine dinucleotide (FAD), participates in fatty acid ß oxidation as a cofactor, which has been confirmed to enhance SCAD activity and expression. However, the role of FAD on hypertensive vascular remodeling is unclear. In this study, we investigated the underlying mechanisms of FAD on vascular remodeling and endothelial homeostasis. MAIN METHODS: Morphological examination of vascular remodeling were analyzed with hematoxylin and eosin (HE) staining, Verhoeff's Van Gieson (EVG) staing, Dihydroethidium (DHE) staining and Sirius red staining. HUVECs apoptotic rate was detected by flow cytometry and HUVECs reactive oxygen species (ROS) was detected by DHE-probe. Enzymatic reactions were used to detect SCAD enzyme activity. The protein level was detected by Western Blots, the mRNA level was detected by quantitative real-time PCR. KEY FINDINGS: In vivo experiments, FAD significantly decreased blood pressure and ameliorated vascular remodeling by increasing SCAD expression, Nitric Oxide (NO) production and reducing ROS production. In vitro experiments, FAD protected against the tBHP induced injury in HUVEC, by increasing the activity of SCAD, increasing the elimination of free fatty acid (FFA), scavenging ROS, reducing apoptotic rate, thereby improving endothelial cell function. SIGNIFICANCE: FAD has a new possibility for preventing and treating hypertensive vascular remodeling.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Activadores de Enzimas / Acil-CoA Deshidrogenasas / Flavina-Adenina Dinucleótido / Remodelación Vascular / Hipertensión Límite: Animals / Humans / Male Idioma: En Revista: Life Sci Año: 2020 Tipo del documento: Article Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Activadores de Enzimas / Acil-CoA Deshidrogenasas / Flavina-Adenina Dinucleótido / Remodelación Vascular / Hipertensión Límite: Animals / Humans / Male Idioma: En Revista: Life Sci Año: 2020 Tipo del documento: Article Pais de publicación: Países Bajos