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Surface modified nanoliposome formulations provide sustained release for 5-FU and increase cytotoxicity on A431 cell line.
Yaman, Ümran; Aslan, Minela; Ozturk, Sukru; Ulubayram, Kezban; Eroglu, Ipek.
Afiliación
  • Yaman Ü; Department of Nanotechnology and Nanomedicine, Institute for Graduate Studies in Science Engineering, Hacettepe University, Ankara, Turkey.
  • Aslan M; Bioengineering Division, Institute for Graduate Studies in Science & Engineering, Hacettepe University, Ankara, Turkey.
  • Ozturk S; Bioengineering Division, Institute for Graduate Studies in Science & Engineering, Hacettepe University, Ankara, Turkey.
  • Ulubayram K; Department of Basic Pharmaceutical Sciences, Faculty of Pharmacy, Hacettepe University, Ankara, Turkey.
  • Eroglu I; Department of Nanotechnology and Nanomedicine, Institute for Graduate Studies in Science Engineering, Hacettepe University, Ankara, Turkey.
Pharm Dev Technol ; 25(10): 1192-1203, 2020 Dec.
Article en En | MEDLINE | ID: mdl-32729757
Malignant melanoma is a type of skin cancer with high risk of metastasis. 5-Fluorouracil is commonly used for treatment of skin cancer, however its penetration through the skin is found to be insufficient in some cases. Therefore, we optimized its pharmacokinetics by fabricating 5- Fluorouracil-loaded nanoliposome formulations modified with Poly-L-lysine coating. 5-Fluorouracil-loaded nanoliposome formulations were prepared using dipalmitoylphosphatidylcholine, dicethylphosphate and cholesterol having encapsulation efficiency of 45 ± 9.61%. The particle size, zeta potential, polydispersity index and encapsulation rate of the prepared formulation was found to be 237.9 ± 0.986 nm, 41.4 ± 1.060 mV, 0.233 ± 0.019 and 88.2 ± 7.85%, respectively. Surface characterization, molecular structure and thermal property illumination of the formulations were performed alongside stability studies. The In-vitro release of 5-FU from Lipo-FU6 and PLL-1 formulations was investigated by dialysis membrane method. Within the first 12 hours, the percentage release of 5-FU from Lipo-FU6 and PLL-1 formulations was observed to be 47.17% and 20.84%, respectively. Moreover, the cytotoxicity study on A431 epidermal carcinoma cell lines has revealed that 5-FU-loaded formulations were toxic to cells unlike the 5-FU free formulations. In conclusion, PLL coated nanoliposome formulations showed a potential to be an effective option for further combined drug/gene therapy applications.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias Cutáneas / Nanopartículas / Fluorouracilo / Antimetabolitos Antineoplásicos Límite: Humans Idioma: En Revista: Pharm Dev Technol Asunto de la revista: FARMACIA Año: 2020 Tipo del documento: Article País de afiliación: Turquía Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias Cutáneas / Nanopartículas / Fluorouracilo / Antimetabolitos Antineoplásicos Límite: Humans Idioma: En Revista: Pharm Dev Technol Asunto de la revista: FARMACIA Año: 2020 Tipo del documento: Article País de afiliación: Turquía Pais de publicación: Reino Unido