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Circadian Rhythms in Hormone-sensitive Lipase in Human Adipose Tissue: Relationship to Meal Timing and Fasting Duration.
Arredondo-Amador, María; Zambrano, Carolina; Kulyté, Agné; Luján, Juán; Hu, Kun; Sánchez de Medina, Fermín; Scheer, Frank A J L; Arner, Peter; Ryden, Mikael; Martínez-Augustin, Olga; Garaulet, Marta.
Afiliación
  • Arredondo-Amador M; Department of Pharmacology, Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd), Instituto de Investigación Biosanitaria ibs, Granada, School of Pharmacy, University of Granada, Granada, Spain.
  • Zambrano C; Department of Physiology, Biomedical Research Institute of Murcia (IMIB)-Arrixaca, University of Murcia, Murcia, Spain.
  • Kulyté A; Endocrinology Unit, Department of Medicine (H7) Huddinge, Karolinska Institutet, Stockholm, Sweden.
  • Luján J; General Surgery Service, University Hospital Virgen de la Arrixaca, Murcia, Spain.
  • Hu K; Division of Sleep and Circadian Disorders, Brigham and Women's Hospital, Boston, Massachusetts; Division of Sleep Medicine, Harvard Medical School, Boston, Massachusetts.
  • Sánchez de Medina F; Department of Pharmacology, Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd), Instituto de Investigación Biosanitaria ibs, Granada, School of Pharmacy, University of Granada, Granada, Spain.
  • Scheer FAJL; Division of Sleep and Circadian Disorders, Brigham and Women's Hospital, Boston, Massachusetts; Division of Sleep Medicine, Harvard Medical School, Boston, Massachusetts.
  • Arner P; Endocrinology Unit, Department of Medicine (H7) Huddinge, Karolinska Institutet, Stockholm, Sweden.
  • Ryden M; Endocrinology Unit, Department of Medicine (H7) Huddinge, Karolinska Institutet, Stockholm, Sweden.
  • Martínez-Augustin O; Department of Biochemistry and Molecular Biology II, School of Pharmacy, Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd), Instituto de Investigación Biosanitaria ibs, Granada, Instituto de Nutrición y Tecnología de los Alimentos José Mataix, University of G
  • Garaulet M; Department of Physiology, Biomedical Research Institute of Murcia (IMIB)-Arrixaca, University of Murcia, Murcia, Spain.
J Clin Endocrinol Metab ; 105(12)2020 12 01.
Article en En | MEDLINE | ID: mdl-32725188
BACKGROUND: Fat mobilization in adipose tissue (AT) has a specific timing. However, circadian rhythms in the activity of the major enzyme responsible for fat mobilization, hormone-sensitive lipase (HSL), have not been demonstrated in humans. OBJECTIVE: To analyze in a cross-sectional study whether there is an endogenous circadian rhythm in HSL activity in human AT ex vivo and whether rhythm characteristics are related to food timing or fasting duration. METHODS: Abdominal AT biopsies were obtained from 18 severely obese participants (age: 46 ± 11 years; body mass index 42 ± 6 kg/m2) who underwent laparoscopic gastric bypass. Twenty-four-hour rhythms of HSL activity and LIPE (HSL transcript in humans) expression in subcutaneous AT were analyzed together with habitual food timing and night fasting duration. RESULTS: HSL activity exhibited a circadian rhythm (P = .023) and reached the maximum value at circadian time 16 (CT) that corresponded to around midnight (relative local clock time. Similarly, LIPE displayed a circadian rhythm with acrophase also at night (P = .0002). Participants with longer night fasting duration >11.20 hours displayed almost double the amplitude (1.91 times) in HSL activity rhythm than those with short duration (P = .013); while habitual early diners (before 21:52 hours) had 1.60 times higher amplitude than late diners (P = .035). CONCLUSIONS: Our results demonstrate circadian rhythms in HSL activity and may lead to a better understanding of the intricate relationships between food timing, fasting duration and body fat regulation.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Tejido Adiposo / Esterol Esterasa / Ayuno / Ritmo Circadiano / Obesidad Tipo de estudio: Diagnostic_studies / Observational_studies / Prevalence_studies / Risk_factors_studies Límite: Adult / Female / Humans / Male / Middle aged Idioma: En Revista: J Clin Endocrinol Metab Año: 2020 Tipo del documento: Article País de afiliación: España Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Tejido Adiposo / Esterol Esterasa / Ayuno / Ritmo Circadiano / Obesidad Tipo de estudio: Diagnostic_studies / Observational_studies / Prevalence_studies / Risk_factors_studies Límite: Adult / Female / Humans / Male / Middle aged Idioma: En Revista: J Clin Endocrinol Metab Año: 2020 Tipo del documento: Article País de afiliación: España Pais de publicación: Estados Unidos