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Isolation and Biophysical Characterisation of Bioactive Polysaccharides from Cucurbita Moschata (Butternut Squash).
Jiwani, Shahwar Imran; Gillis, Richard B; Besong, David; Almutairi, Fahad; Erten, Tayyibe; Kök, M Samil; Harding, Stephen E; Paulsen, Berit S; Adams, Gary G.
Afiliación
  • Jiwani SI; Queen's Medical Centre, Faculty of Medicine and Health Sciences, University of Nottingham, Clifton Boulevard, Nottingham NG7 2UH, UK.
  • Gillis RB; Queen's Medical Centre, Faculty of Medicine and Health Sciences, University of Nottingham, Clifton Boulevard, Nottingham NG7 2UH, UK.
  • Besong D; Solar and Photovoltaics Engineering Center, King Abdullah University of Science and Technology, Thuwal, Makkah 23955-6900, Saudi Arabia.
  • Almutairi F; Department of Biochemistry, Faculty of Science, University of Tabuk, P.O. Box 741, Tabuk 71491, Saudi Arabia.
  • Erten T; Department of Nutrition and Dietetics, Faculty of Health Sciences, Bayburt University, 69000 Bayburt, Turkey.
  • Kök MS; Department of Food Engineering, Faculty of Engineering & Architecture, Abant Izzet Baysal University, Gölköy, 14300 Bolu, Turkey.
  • Harding SE; National Centre for Macromolecular Hydrodynamics (NCMH), School of Biosciences, Sutton Bonington Campus, The University of Nottingham, Sutton Bonington, Leicestershire LE12 5RD, UK.
  • Paulsen BS; Department of Pharmaceutical Chemistry, School of Pharmacy, Section Pharmacognosy, University of Oslo, PB 1068, Blindern, N-0316 Oslo, Norway.
  • Adams GG; Queen's Medical Centre, Faculty of Medicine and Health Sciences, University of Nottingham, Clifton Boulevard, Nottingham NG7 2UH, UK.
Polymers (Basel) ; 12(8)2020 Jul 24.
Article en En | MEDLINE | ID: mdl-32722155
Cucurbits are plants that have been used frequently as functional foods. This study includes the extraction, isolation, and characterisation of the mesocarp polysaccharide of Cucurbita moschata. The polysaccharide component was purified by gel filtration into three fractions (NJBTF1, NJBTF2, and NJBTF3) of different molecular weights. Characterisation includes the hydrodynamic properties, identification of monosaccharide composition, and bioactivity. Sedimentation velocity also indicated the presence of small amounts of additional discrete higher molecular weight components even after fractionation. Sedimentation equilibrium revealed respective weight average molecular weights of 90, 31, and 19 kDa, with the higher fractions (NJBTF1 and NJBTF2) indicating a tendency to self-associate. Based on the limited amount of data (combinations of 3 sets of viscosity and sedimentation data corresponding to the 3 fractions), HYDFIT indicates an extended, semi-flexible coil conformation. Of all the fractions obtained, NJBTF1 showed the highest bioactivity. All fractions contained galacturonic acid and variable amounts of neutral sugars. To probe further, the extent of glycosidic linkages in NJBTF1 was estimated using gas chromatography-mass spectrometry (GCMS), yielding a high galacturonic acid content (for pectin polysaccharide) and the presence of fructans-the first evidence of fructans (levan) in the mesocarp. Our understanding of the size and structural flexibility together with the high bioactivity suggests that the polysaccharide obtained from C. moschata has the potential to be developed into a therapeutic agent.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Prognostic_studies Idioma: En Revista: Polymers (Basel) Año: 2020 Tipo del documento: Article Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Prognostic_studies Idioma: En Revista: Polymers (Basel) Año: 2020 Tipo del documento: Article Pais de publicación: Suiza