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Chemoenzymatic synthesis of arabinomannan (AM) glycoconjugates as potential vaccines for tuberculosis.
Li, Zhihao; Bavaro, Teodora; Tengattini, Sara; Bernardini, Roberta; Mattei, Maurizio; Annunziata, Francesca; Cole, Richard B; Zheng, Changping; Sollogoub, Matthieu; Tamborini, Lucia; Terreni, Marco; Zhang, Yongmin.
Afiliación
  • Li Z; Sorbonne Université, CNRS, Institut Parisien de Chimie Moléculaire, UMR 8232, 4 Place Jussieu, 75005, Paris, France.
  • Bavaro T; Drug Sciences Department, University of Pavia, Viale Taramelli 12, 27100, Pavia, Italy.
  • Tengattini S; Drug Sciences Department, University of Pavia, Viale Taramelli 12, 27100, Pavia, Italy.
  • Bernardini R; Italy Centro Servizi Interdipartimentale - STA, University of Rome "Tor Vergata", Rome, Italy.
  • Mattei M; Italy Centro Servizi Interdipartimentale - STA, University of Rome "Tor Vergata", Rome, Italy; Department of Biology, University of Rome "Tor Vergata", Rome, Italy.
  • Annunziata F; Department of Pharmaceutical Sciences, University of Milan, via Mangiagalli 25, 20133, Milan, Italy.
  • Cole RB; Sorbonne Université, CNRS, Institut Parisien de Chimie Moléculaire, UMR 8232, 4 Place Jussieu, 75005, Paris, France.
  • Zheng C; Sorbonne Université, CNRS, Institut Parisien de Chimie Moléculaire, UMR 8232, 4 Place Jussieu, 75005, Paris, France.
  • Sollogoub M; Sorbonne Université, CNRS, Institut Parisien de Chimie Moléculaire, UMR 8232, 4 Place Jussieu, 75005, Paris, France.
  • Tamborini L; Department of Pharmaceutical Sciences, University of Milan, via Mangiagalli 25, 20133, Milan, Italy.
  • Terreni M; Drug Sciences Department, University of Pavia, Viale Taramelli 12, 27100, Pavia, Italy. Electronic address: marco.terreni@unipv.it.
  • Zhang Y; Sorbonne Université, CNRS, Institut Parisien de Chimie Moléculaire, UMR 8232, 4 Place Jussieu, 75005, Paris, France; Key Laboratory of Tropical Medicinal Resource Chemistry of Ministry of Education, College of Chemistry & Chemical Engineering, Hainan Normal University, Haikou 571158, China. Elec
Eur J Med Chem ; 204: 112578, 2020 Oct 15.
Article en En | MEDLINE | ID: mdl-32717482
Mycobacteria infection resulting in tuberculosis (TB) is one of the top ten leading causes of death worldwide in 2018, and lipoarabinomannan (LAM) has been confirmed to be the most important antigenic polysaccharide on the TB cell surface. In this study, a convenient synthetic method has been developed for synthesizing three branched oligosaccharides derived from LAM, in which a core building block was prepared by enzymatic hydrolysis in flow chemistry with excellent yield. After several steps of glycosylations, the obtained oligosaccharides were conjugated with recombinant human serum albumin (rHSA) and the ex-vivo ELISA tests were performed using serum obtained from several TB-infected patients, in order to evaluate the affinity of the glycoconjugate products for the human LAM-antibodies. The evaluation results are positive, especially compound 21 that exhibited excellent activity which could be considered as a lead compound for the future development of a new glycoconjugated vaccine against TB.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Tuberculosis / Glicoconjugados / Vacunas Bacterianas / Mananos Límite: Humans Idioma: En Revista: Eur J Med Chem Año: 2020 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Francia
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Tuberculosis / Glicoconjugados / Vacunas Bacterianas / Mananos Límite: Humans Idioma: En Revista: Eur J Med Chem Año: 2020 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Francia