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Metabolic engineering considerations for the heterologous expression of xylose-catabolic pathways in Saccharomyces cerevisiae.
Jeong, Deokyeol; Oh, Eun Joong; Ko, Ja Kyong; Nam, Ju-Ock; Park, Hee-Soo; Jin, Yong-Su; Lee, Eun Jung; Kim, Soo Rin.
Afiliación
  • Jeong D; School of Food Science and Biotechnology, Kyungpook National University, Daegu, Republic of Korea.
  • Oh EJ; Renewable and Sustainable Energy Institute (RASEI), University of Colorado Boulder, Boulder, Colorado, United States of America.
  • Ko JK; Clean Energy Research Center, Korea Institute of Science and Technology (KIST), Seoul, Republic of Korea.
  • Nam JO; School of Food Science and Biotechnology, Kyungpook National University, Daegu, Republic of Korea.
  • Park HS; School of Food Science and Biotechnology, Kyungpook National University, Daegu, Republic of Korea.
  • Jin YS; Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, Illinois, United States of America.
  • Lee EJ; Department of Food Science and Human Nutrition, University of Illinois at Urbana-Champaign, Urbana, Illinois, United States of America.
  • Kim SR; Department of Chemical Engineering, School of Applied Chemical Engineering, Kyungpook National University, Daegu, Republic of Korea.
PLoS One ; 15(7): e0236294, 2020.
Article en En | MEDLINE | ID: mdl-32716960
Xylose, the second most abundant sugar in lignocellulosic biomass hydrolysates, can be fermented by Saccharomyces cerevisiae expressing one of two heterologous xylose pathways: a xylose oxidoreductase pathway and a xylose isomerase pathway. Depending on the type of the pathway, its optimization strategies and the fermentation efficiencies vary significantly. In the present study, we constructed two isogenic strains expressing either the oxidoreductase pathway (XYL123) or the isomerase pathway (XI-XYL3), and delved into simple and reproducible ways to improve the resulting strains. First, the strains were subjected to the deletion of PHO13, overexpression of TAL1, and adaptive evolution, but those individual approaches were only effective in the XYL123 strain but not in the XI-XYL3 strain. Among other optimization strategies of the XI-XYL3 strain, we found that increasing the copy number of the xylose isomerase gene (xylA) is the most promising but yet preliminary strategy for the improvement. These results suggest that the oxidoreductase pathway might provide a simpler metabolic engineering strategy than the isomerase pathway for the development of efficient xylose-fermenting strains under the conditions tested in the present study.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Saccharomyces cerevisiae / Xilosa / Redes y Vías Metabólicas / Ingeniería Metabólica Idioma: En Revista: PLoS One Asunto de la revista: CIENCIA / MEDICINA Año: 2020 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Saccharomyces cerevisiae / Xilosa / Redes y Vías Metabólicas / Ingeniería Metabólica Idioma: En Revista: PLoS One Asunto de la revista: CIENCIA / MEDICINA Año: 2020 Tipo del documento: Article Pais de publicación: Estados Unidos