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Characteristics of Biopeptides Released In Silico from Collagens Using Quantitative Parameters.
Iwaniak, Anna; Minkiewicz, Piotr; Pliszka, Monika; Mogut, Damir; Darewicz, Malgorzata.
Afiliación
  • Iwaniak A; University of Warmia and Mazury in Olsztyn, Faculty of Food Science, Chair of Food Biochemistry, Pl. Cieszynski 1, 10-719 Olsztyn-Kortowo, Poland.
  • Minkiewicz P; University of Warmia and Mazury in Olsztyn, Faculty of Food Science, Chair of Food Biochemistry, Pl. Cieszynski 1, 10-719 Olsztyn-Kortowo, Poland.
  • Pliszka M; University of Warmia and Mazury in Olsztyn, Faculty of Food Science, Chair of Food Biochemistry, Pl. Cieszynski 1, 10-719 Olsztyn-Kortowo, Poland.
  • Mogut D; University of Warmia and Mazury in Olsztyn, Faculty of Food Science, Chair of Food Biochemistry, Pl. Cieszynski 1, 10-719 Olsztyn-Kortowo, Poland.
  • Darewicz M; University of Warmia and Mazury in Olsztyn, Faculty of Food Science, Chair of Food Biochemistry, Pl. Cieszynski 1, 10-719 Olsztyn-Kortowo, Poland.
Foods ; 9(7)2020 Jul 21.
Article en En | MEDLINE | ID: mdl-32708318
The potential of collagens to release biopeptides was evaluated using the BIOPEP-UWM-implemented quantitative criteria including the frequency of the release of fragments with a given activity by selected enzyme(s) (AE), relative frequency of release of fragments with a given activity by selected enzyme(s) (W), and the theoretical degree of hydrolysis (DHt). Cow, pig, sheep, chicken, duck, horse, salmon, rainbow trout, goat, rabbit, and turkey collagens were theoretically hydrolyzed using: stem bromelain, ficin, papain, pepsin, trypsin, chymotrypsin, pepsin+trypsin, and pepsin+trypsin+chymotrypsin. Peptides released from the collagens having comparable AE and W were estimated for their likelihood to be bioactive using PeptideRanker Score. The collagens tested were the best sources of angiotensin I-converting enzyme (ACE) and dipeptidyl peptidase IV (DPP-IV) inhibitors. AE and W values revealed that pepsin and/or trypsin were effective producers of such peptides from the majority of the collagens examined. Then, the SwissTargetPrediction program was used to estimate the possible interactions of such peptides with enzymes and proteins, whereas ADMETlab was applied to evaluate their safety and drug-likeness properties. Target prediction revealed that the collagen-derived peptides might interact with several human proteins, especially proteinases, but with relatively low probability. In turn, their bioactivity may be limited by their short half-life in the body.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Prognostic_studies Idioma: En Revista: Foods Año: 2020 Tipo del documento: Article País de afiliación: Polonia Pais de publicación: Suiza

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Prognostic_studies Idioma: En Revista: Foods Año: 2020 Tipo del documento: Article País de afiliación: Polonia Pais de publicación: Suiza