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Development of an immunochromatographic lateral flow dipstick for the detection of Mycobacterium tuberculosis 16 kDa antigen (Mtb-strip).
Mohd Amiruddin, M N; Ang, Geik Yong; Yu, Choo Yee; Falero-Diaz, G; Otero, O; Reyes, F; Camacho, F; Chin, K L; Sarmiento, M E; Norazmi, M N; Acosta, A; Yean Yean, C.
Afiliación
  • Mohd Amiruddin MN; School of Health Sciences, Universiti Sains Malaysia (USM), Kota Bharu, Malaysia.
  • Ang GY; School of Medical of Sciences, Universiti Sains Malaysia (USM), Kota Bharu, Malaysia.
  • Yu CY; School of Medical of Sciences, Universiti Sains Malaysia (USM), Kota Bharu, Malaysia.
  • Falero-Diaz G; University of Miami, Miami, USA.
  • Otero O; Center of Molecular Immunology, La Habana, Cuba.
  • Reyes F; School of Biological Sciences, University of Concepcion, Concepcion, Chile.
  • Camacho F; School of Biological Sciences, University of Concepcion, Concepcion, Chile.
  • Chin KL; Faculty of Medicine and Health Sciences, Universiti Malaysia Sabah (UMS), Kota Kinabalu, Sabah, Malaysia.
  • Sarmiento ME; School of Health Sciences, Universiti Sains Malaysia (USM), Kota Bharu, Malaysia.
  • Norazmi MN; School of Health Sciences, Universiti Sains Malaysia (USM), Kota Bharu, Malaysia. Electronic address: norazmimn@usm.my.
  • Acosta A; School of Health Sciences, Universiti Sains Malaysia (USM), Kota Bharu, Malaysia. Electronic address: armando@usm.my.
  • Yean Yean C; School of Medical of Sciences, Universiti Sains Malaysia (USM), Kota Bharu, Malaysia. Electronic address: yychan@usm.my.
J Microbiol Methods ; 176: 106003, 2020 09.
Article en En | MEDLINE | ID: mdl-32702386
Mycobacterium tuberculosis (Mtb) is a pathogenic bacterium that causes tuberculosis (TB). This contagious disease remains a severe health problem in the world. The disease is transmitted via inhalation of airborne droplets carrying Mtb from TB patients. Early detection of the disease is vital to prevent transmission of the infection to people in close contact with the patients. To date, there is a need of a simple, rapid, sensitive and specific diagnostic test for TB. Previous studies showed the potential of Mtb 16 kDa antigen (Ag16) in TB diagnosis. In this study, lateral flow immunoassay, also called simple strip immunoassay or immunochromatographic test (ICT) for detection of Ag16 was developed (Mtb-strip) and assessed as a potential rapid TB diagnosis method. A monoclonal antibody against Ag16 was optimized as the capturing and detection antibody on the Mtb-strip. Parameters affecting the performance of the Mtb-strip were also optimized before a complete prototype was developed. Analytical sensitivity showed that Mtb-strip was capable to detect as low as 125 ng of purified Ag16. The analytical sensitivity of Mtb-strip suggests its potential usefulness in different clinical applications.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas Bacterianas / Tuberculosis / Cromatografía de Afinidad / Pruebas Diagnósticas de Rutina / Antígenos Bacterianos Tipo de estudio: Diagnostic_studies / Screening_studies Límite: Humans Idioma: En Revista: J Microbiol Methods Año: 2020 Tipo del documento: Article País de afiliación: Malasia Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas Bacterianas / Tuberculosis / Cromatografía de Afinidad / Pruebas Diagnósticas de Rutina / Antígenos Bacterianos Tipo de estudio: Diagnostic_studies / Screening_studies Límite: Humans Idioma: En Revista: J Microbiol Methods Año: 2020 Tipo del documento: Article País de afiliación: Malasia Pais de publicación: Países Bajos