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Force-exerting perpendicular lateral protrusions in fibroblastic cell contraction.
Padhi, Abinash; Singh, Karanpreet; Franco-Barraza, Janusz; Marston, Daniel J; Cukierman, Edna; Hahn, Klaus M; Kapania, Rakesh K; Nain, Amrinder S.
Afiliación
  • Padhi A; Department of Mechanical Engineering, Virginia Tech, Blacksburg, VA, USA.
  • Singh K; Department of Aerospace and Ocean Engineering, Virginia Tech, Blacksburg, VA, USA.
  • Franco-Barraza J; Cancer Biology Program, Marvin & Concetta Greenberg Pancreatic Cancer Institute, Fox Chase Cancer Center, Philadelphia, PA, USA.
  • Marston DJ; Department of Pharmacology, The University of North Carolina at Chapel Hill, Chapel Hill, NC, 27599, USA.
  • Cukierman E; Cancer Biology Program, Marvin & Concetta Greenberg Pancreatic Cancer Institute, Fox Chase Cancer Center, Philadelphia, PA, USA. Edna.Cukierman@fccc.edu.
  • Hahn KM; Department of Pharmacology, The University of North Carolina at Chapel Hill, Chapel Hill, NC, 27599, USA.
  • Kapania RK; Department of Aerospace and Ocean Engineering, Virginia Tech, Blacksburg, VA, USA.
  • Nain AS; Department of Mechanical Engineering, Virginia Tech, Blacksburg, VA, USA. nain@vt.edu.
Commun Biol ; 3(1): 390, 2020 07 21.
Article en En | MEDLINE | ID: mdl-32694539
Aligned extracellular matrix fibers enable fibroblasts to undergo myofibroblastic activation and achieve elongated shapes. Activated fibroblasts are able to contract, perpetuating the alignment of these fibers. This poorly understood feedback process is critical in chronic fibrosis conditions, including cancer. Here, using fiber networks that serve as force sensors, we identify "3D perpendicular lateral protrusions" (3D-PLPs) that evolve from lateral cell extensions named twines. Twines originate from stratification of cyclic-actin waves traversing the cell and swing freely in 3D to engage neighboring fibers. Once engaged, a lamellum forms and extends multiple secondary twines, which fill in to form a sheet-like PLP, in a force-entailing process that transitions focal adhesions to activated (i.e., pathological) 3D-adhesions. The specific morphology of PLPs enables cells to increase contractility and force on parallel fibers. Controlling geometry of extracellular networks confirms that anisotropic fibrous environments support 3D-PLP formation and function, suggesting an explanation for cancer-associated desmoplastic expansion.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Citoesqueleto / Adhesiones Focales / Matriz Extracelular / Neoplasias Límite: Humans Idioma: En Revista: Commun Biol Año: 2020 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Citoesqueleto / Adhesiones Focales / Matriz Extracelular / Neoplasias Límite: Humans Idioma: En Revista: Commun Biol Año: 2020 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido