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Detection and molecular characterization of porcine enterovirus G15 and teschovirus from India.
Sawant, Pradeep Mahadev; Atre, Nitin; Kulkarni, Abhijeet; Gopalkrishna, Varanasi.
Afiliación
  • Sawant PM; Enteric Virus Group, ICMR-National Institute of Virology, 20-A, Ambedkar Road, Pune 411001, Maharashtra, India.
  • Atre N; Bioinformatics Group, ICMR-National Institute of Virology (Pashan Campus), Pune 411021, Maharashtra, India.
  • Kulkarni A; Bioinformatics Centre, Savitribai Phule Pune University, Pune 411007, Maharashtra, India.
  • Gopalkrishna V; Enteric Virus Group, ICMR-National Institute of Virology, 20-A, Ambedkar Road, Pune 411001, Maharashtra, India.
Pathog Dis ; 78(5)2020 07 01.
Article en En | MEDLINE | ID: mdl-32691821
Porcine enterovirus G (EV-G) and teschovirus (PTV) generally cause asymptomatic infections. Although both viruses have been reported from various countries, they are rarely detected from India. To detect these viruses in Western India, fecal samples (n = 26) of diarrheic piglets aged below three months from private pig farms near Pune (Maharashtra) were collected. The samples were screened by reverse transcription-polymerase chain reaction using conserved enterovirus specific primers from 5' untranslated region. For genetic characterization of detected EV-G strain, nearly complete genome, and for PTV, partial VP1 gene were sequenced. EV-G strain showed the highest identity in a VP1 gene at nucleotide (78.61%) and amino acid (88.65%) level with EV-G15, prototype strain. However, its complete genome was homologous with the nucleotide (78.38% identity) and amino acid (91.24% identity) level to Ishi-Ka2 strain (LC316832), unassigned EV-G genotype detected from Japan. The nearly complete genome of EV-G15 consisted of 7398 nucleotides excluding the poly(A) tail and has an open reading frame that encodes a 2170 amino acid polyprotein. Genetic analysis of the partial VP1 gene of teschovirus identified porcine teschovirus 4 (PTV-4) and putative PTV-17 genotype. To the best of our knowledge, this is the first report on nearly full genome characterization of EV-G15, and detection of PTV-4 and putative PTV-17 genotypes from India. Further, detection and characterization of porcine enteroviruses are needed for a comprehensive understanding of their genetic diversity and their association with symptomatic infections from other geographical regions of India.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enterovirus Porcinos / Teschovirus Tipo de estudio: Diagnostic_studies Límite: Animals País/Región como asunto: Asia Idioma: En Revista: Pathog Dis Año: 2020 Tipo del documento: Article País de afiliación: India Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enterovirus Porcinos / Teschovirus Tipo de estudio: Diagnostic_studies Límite: Animals País/Región como asunto: Asia Idioma: En Revista: Pathog Dis Año: 2020 Tipo del documento: Article País de afiliación: India Pais de publicación: Estados Unidos